Resolving physical interactions between bacteria and nanotopographies with focused ion beam scanning electron microscopy.

Autor:	Jenkins J; Bristol Dental School, University of Bristol, Bristol, UK., Ishak MI; Bristol Dental School, University of Bristol, Bristol, UK.; Faculty of Engineering Technology, Universiti Malaysia Perlis, Malaysia., Eales M; Bristol Dental School, University of Bristol, Bristol, UK., Gholinia A; School of Materials Science, University of Manchester, Manchester, UK., Kulkarni S; Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, Hamburg 22607, Germany., Keller TF; Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, Hamburg 22607, Germany.; Physics Department, University of Hamburg, Hamburg, Germany., May PW; School of Chemistry, University of Bristol, Bristol, UK., Nobbs AH; Bristol Dental School, University of Bristol, Bristol, UK., Su B; Bristol Dental School, University of Bristol, Bristol, UK.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2021 Jul 07; Vol. 24 (7), pp. 102818. Date of Electronic Publication: 2021 Jul 07 (Print Publication: 2021).
DOI:	10.1016/j.isci.2021.102818
Abstrakt:	To robustly assess the antibacterial mechanisms of nanotopographies, it is critical to analyze the bacteria-nanotopography adhesion interface. Here, we utilize focused ion beam milling combined with scanning electron microscopy to generate three-dimensional reconstructions of Staphylococcus aureus or Escherichia coli interacting with nanotopographies. For the first time, 3D morphometric analysis has been exploited to quantify the intrinsic contact area between each nanostructure and the bacterial envelope, providing an objective framework from which to derive the possible antibacterial mechanisms of synthetic nanotopographies. Surfaces with nanostructure densities between 36 and 58 per μm 2 and tip diameters between 27 and 50 nm mediated envelope deformation and penetration, while surfaces with higher nanostructure densities (137 per μm 2 ) induced envelope penetration and mechanical rupture, leading to marked reductions in cell volume due to cytosolic leakage. On nanotopographies with densities of 8 per μm 2 and tip diameters greater than 100 nm, bacteria predominantly adhered between nanostructures, resulting in cell impedance. Competing Interests: The authors declare no competing interests. (© 2021 The Authors.)
Databáze:	MEDLINE
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