Diagnostic Performances of Three Rapid Diagnostic Tests for Detecting HIV Infections in Mali.

Autor: Kone B; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Sarro YS; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Baya B; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Dabitao D; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Coulibaly N; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Wague M; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Diarra B; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Guindo O; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Sanogo M; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Togo AC; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Kone A; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Goita D; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Diabate S; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Kodio O; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Belson M; National Institute of Allergic and Infectious Diseases (NIAID), Bethesda, Maryland, USA., Dao S; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Orsega S; National Institute of Allergic and Infectious Diseases (NIAID), Bethesda, Maryland, USA., Murphy RL; Northwestern University, Chicago, Illinois, USA., Diallo S; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Doumbia S; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali., Siddiqui S; National Institute of Allergic and Infectious Diseases (NIAID), Bethesda, Maryland, USA., Maiga M; University Clinical Research Center (UCRC) - SEREFO Laboratory, University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali.; Northwestern University, Chicago, Illinois, USA.
Jazyk: angličtina
Zdroj: Infectious diseases diagnosis & treatment [Infect Dis Diagn Treat] 2019; Vol. 3 (1). Date of Electronic Publication: 2019 Dec 18.
Abstrakt: Diagnosis of HIV infections in resource-limited countries like Mali is based on Rapid Diagnostic Tests (RDTs). The RDTs are diagnostic assays designed for use at the Point-Of-Care (POC), which is quick, cost-effective and easy to perform. However, in these countries, the tests are commonly used without any initial evaluation or monitoring of their performance despite high levels of HIV strain diversity and rapid evolution of the virus. In this study, the reliability and accuracy of HIV RDTs (Determine ™ , Multispot ™ , SD Bioline ™ ) used in Mali, where HIV-1 and HIV-2 co-exist, were evaluated from August 2004 to November 2017. A total of 1303 samples from new HIV-suspect patients in Bamako were tested for HIV-1 and HIV-2 using the RDT Determine ™ , followed by ELISA and Western Blot (WB). The Determine ™ test showed a robust diagnostic sensitivity of 98.7% [CI 95: 97.59-99.37] and a diagnostic specificity of 99.2% [CI 95: 98.22-99.67]. The Multispot ™ assay showed a diagnostic sensitivity of 98.77% [CI 95: 97.59-99.37] and a diagnostic specificity of 99.2% [CI 95: 98.22-99.67]. The diagnostic sensitivity and specificity of SD Bioline ™ HIV-1/2 were 100% [CI 95:72.25-100] and 88.89% [CI 95: 56.50- 98.71], respectively. These data indicate excellent performance for HIV RDTs in Mali and we recommend the use of Determine ™ HIV-1/2 for HIV screening and Multispot™ for discriminating HIV-2 from HIV-1 infections.
Databáze: MEDLINE
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