Emergence and Clonal Spread of CTX-M-65-Producing Escherichia coli From Retail Meat in Portugal.

Autor: Leão C; Laboratory of Bacteriology and Mycology, National Institute of Agrarian and Veterinary Research (INIAV, IP), Oeiras, Portugal.; MED - Mediterranean Institute for Agriculture, Environment and Development, Évora, Portugal., Clemente L; Laboratory of Bacteriology and Mycology, National Institute of Agrarian and Veterinary Research (INIAV, IP), Oeiras, Portugal.; Faculty of Veterinary Science, CIISA- Centre for Interdisciplinary Research in Animal Health, Lisbon, Portugal., Moura L; Laboratory of Bacteriology and Mycology, National Institute of Agrarian and Veterinary Research (INIAV, IP), Oeiras, Portugal.; Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal., Seyfarth AM; EURL-AR, European Reference Laboratory for Antimicrobial Resistance, Technical University of Denmark (DTU), National Food Institute, Lyngby, Denmark., Hansen IM; EURL-AR, European Reference Laboratory for Antimicrobial Resistance, Technical University of Denmark (DTU), National Food Institute, Lyngby, Denmark., Hendriksen RS; EURL-AR, European Reference Laboratory for Antimicrobial Resistance, Technical University of Denmark (DTU), National Food Institute, Lyngby, Denmark., Amaro A; Laboratory of Bacteriology and Mycology, National Institute of Agrarian and Veterinary Research (INIAV, IP), Oeiras, Portugal.
Jazyk: angličtina
Zdroj: Frontiers in microbiology [Front Microbiol] 2021 Jul 20; Vol. 12, pp. 653595. Date of Electronic Publication: 2021 Jul 20 (Print Publication: 2021).
DOI: 10.3389/fmicb.2021.653595
Abstrakt: The emergence and dissemination of resistance to third- and fourth-generation cephalosporins among Enterobacteriaceae from different sources impose a global public health threat. Here, we characterized by whole-genome sequencing four Escherichia coli strains harboring the bla CTX-M-65 gene identified among 49 isolates from beef and pork collected at retail. The genomic content was determined using the Center for Genomic Epidemiology web tools. Additionally, the prediction and reconstruction of plasmids were conducted, the genetic platform of the bla CTX-M-65 genes was investigated, and phylogenetic analysis was carried out using 17 other genomes with the same sequence type and harboring the bla CTX-M-65 gene. All strains harbored bla CTX-M-65 , bla OXA-1 , and bla TEM-1B , and one also carried the bla SHV-12 gene. Other resistance genes, namely, qnrS2 , aac(6')-Ib-c , dfrA14 , sul2 , tetA , and mphA , were present in all the genomes; the mcr -1.1 gene was identified in the colistin-resistant strains. They belong to sequence type 2179, phylogenetic group B1, and serotype O9:H9 and carried plasmids IncI, IncFIC(FII), and IncFIB. All strains share an identical genetic environment with IS 903 and IS Ecp1 flanking the bla CTX-M-65 gene. It seems likely that the bla CTX-M-65 gene is located in the chromosome in all isolates based on deep in silico analysis. Our findings showed that the strains are clonally related and belong to two sub-lineages. This study reports the emergence of CTX-M-65-producing E. coli in Portugal in food products of animal origin. The chromosomal location of the bla CTX-M-65 gene may ensure a stable spread of resistance in the absence of selective pressure.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Leão, Clemente, Moura, Seyfarth, Hansen, Hendriksen and Amaro.)
Databáze: MEDLINE
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