Analgesic Effect of Ziziphus abyssinica Involves Inhibition of Inflammatory Mediators and Modulation of K ATP Channels, Opioidergic and Nitrergic Pathways.

Autor: Henneh IT; School of Pharmacy and Pharmaceutical Sciences, University of Cape Coast, Cape Coast, Ghana., Armah FA; Department of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Cape Coast, Ghana., Ameyaw EO; School of Pharmacy and Pharmaceutical Sciences, University of Cape Coast, Cape Coast, Ghana., Biney RP; School of Pharmacy and Pharmaceutical Sciences, University of Cape Coast, Cape Coast, Ghana., Obese E; School of Pharmacy and Pharmaceutical Sciences, University of Cape Coast, Cape Coast, Ghana., Boakye-Gyasi E; Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana., Adakudugu EA; School of Pharmacy and Pharmaceutical Sciences, University of Cape Coast, Cape Coast, Ghana., Ekor M; Department of Pharmacology, School of Medical Sciences, University of Cape Coast, Cape Coast, Ghana.
Jazyk: angličtina
Zdroj: Frontiers in pharmacology [Front Pharmacol] 2021 Jul 20; Vol. 12, pp. 714722. Date of Electronic Publication: 2021 Jul 20 (Print Publication: 2021).
DOI: 10.3389/fphar.2021.714722
Abstrakt: The diversity offered by natural products has timelessly positioned them as a good source for novel therapeutics for the management of diverse medical conditions, including pain. This study evaluated hydro-ethanolic root bark extract of Ziziphus abyssinica (ZAE) as well as β-amyrin and polpunonic acid isolated from the plant for analgesic property. The study also investigated the mechanism responsible for this action in the extract. The antinociceptive potential of ZAE (30, 100, and 300 mg/kg, p. o .) was assessed using the tail-immersion test (TIT), acetic acid-induced writhing test (AAT), and formalin test (FT). The extract's effect on acute and chronic musculoskeletal pain was also assessed by administering carrageenan unilaterally into the rat gastrocnemius muscles and measuring pain at 12 h and 10 days for acute and chronic pain respectively. The involvement of pro-inflammatory mediators (prostaglandin E 2 , bradykinin, TNF-α, and IL-1β) was assessed. The possible pathways mediating the observed analgesic effect of ZAE were further assessed using the antagonists: naloxone, glibenclamide, N G -L-nitro-arginine methyl ester (L-NAME), atropine, nifedipine, and yohimbine in the FT. Also the analgesic effect of two triterpenoid compounds, β-amyrin and polpunonic acid, previously isolated from the plant was assessed using the TIT. The anti-nociceptive activity of ZAE was demonstrated in the TIT by the significant ( p < 0.05) increase in tail withdrawal threshold in ZAE-treated mice. ZAE also markedly reduced writhing and paw licking responses in both AAT and FT and significantly ( p < 0.05) attenuated both acute and chronic musculoskeletal pain. ZAE also significantly reversed hyperalgesia induced by intraplantar injection of PGE 2 , bradykinin, TNF-α, and IL-1β. Furthermore, data revealed the involvement of opioidergic, ATP-sensitive K + channels and NO-cGMP pathways in the analgesic effect of ZAE. Both β-amyrin and polpunonic acid exhibited analgesic activity in the tail suspension test. Our study demonstrates ZAE as an important source of new therapeutic agents for pain management.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Henneh, Armah, Ameyaw, Biney, Obese, Boakye-Gyasi, Adakudugu and Ekor.)
Databáze: MEDLINE
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