Fibrinolytic Enzymes From Extremophilic Microorganisms in the Development of New Thrombolytic Therapies: Technological Prospecting.

Autor:	Soares Bispo JR; Laboratório de Microbiologia, Imunologia e Parasitologia, Complexo de Ciencias Medicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Brazil., de Oliveira Lima IG; Laboratório de Microbiologia, Imunologia e Parasitologia, Complexo de Ciencias Medicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Brazil., da Silva MB; Laboratório de Microbiologia, Imunologia e Parasitologia, Complexo de Ciencias Medicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Brazil., de Oliveira Feitosa A; Laboratório de Microbiologia, Imunologia e Parasitologia, Complexo de Ciencias Medicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Brazil., Dos Santos ACM; Laboratório de Microbiologia, Imunologia e Parasitologia, Complexo de Ciencias Medicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Brazil., Alexandre Moreira MS; Laboratorio de Farmacologia e Imunologia, Instituto de Ciencias Biologicas e da Saude, Universidade Federal de Alagoas, 57072-900, Maceio, AL, Brazil., Zambrano Passarini MR; Universidade Federal da Integracao Latino-Americana, 85870-650, Foz Do Iguaçu, PR, Brazil., Saraiva Câmara PEA; Departamento de Botanica, Campus Darcy Ribeiro, Universidade de Brasília, Asa Norte, s/n, 70910-900, Brasília, DF, Brazil., Rosa LH; Departamento de Microbiologia, Universidade Federal de Minas Gerais, 31270-901, Belo Horizonte, MG, Brazil., Oliveira VM; Divisao de Recursos Microbianos, Centro Pluridisciplinar de Pesquisas Quimicas, Biologicas e Agricolas. Universidade Estadual de Campinas, UNICAMP, 13148-218, Paulinia, SP, Brazil., de Queiroz AC; Laboratório de Microbiologia, Imunologia e Parasitologia, Complexo de Ciencias Medicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Brazil., Fernandes Duarte AW; Laboratório de Microbiologia, Imunologia e Parasitologia, Complexo de Ciencias Medicas e Enfermagem, Campus Arapiraca, Universidade Federal de Alagoas, 57309-005, Arapiraca, AL, Brazil.
Jazyk:	angličtina
Zdroj:	Recent patents on biotechnology [Recent Pat Biotechnol] 2021; Vol. 15 (3), pp. 169-183.
DOI:	10.2174/1872208315666210805154713
Abstrakt:	Extremophilic microorganisms from a wide variety of extreme natural environments have been researched, and many biotechnological applications have been carried out, due to their capacity to produce biomolecules resistant to extreme conditions, such as fibrinolytic proteases. The search for new fibrinolytic enzymes is important in the development of new therapies against cardiovascular diseases. This article aimed to evaluate the patents filed about protease with fibrinolytic activity produced by extremophilic microorganisms whose use is aimed at the development of new drugs for the treatment of cardiovascular diseases. The prospecting was carried out using data on deposits and patent concessions made available on the technological bases: European Patent Office (EPO), United States Patent and Trademark Office (USPTO), World Intellectual Property Organization (WIPO), Instituto Nacional de Propriedade Industrial - Brazil (INPI), The LENS and Patent Inspiration. The International Patent Classification and subclasses and groups for each document were also evaluated. Although 382 patents were selected using terms related to extreme environments, such as "thermophile" and "acidophiles", few were related to clinical use and were mainly performed using Bacillus subtilis and Streptomyces megasporus strains. A highlight of nattokinase was produced by Bacillus subtilis GDN and actinokinase by Streptomyces megasporus SD5. The low number of patents on enzymes with this profile (extreme environments) revealed a little-explored field, promising in the development of new microbial thrombolytic drugs, such as fibrinolytic enzymes with less adverse effects. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu