Evaluation of interference from hemoglobin C, D, E and S traits on measurements of hemoglobin A1c by fifteen methods.

Autor: Rohlfing C; Department of Pathology and Anatomical Sciences, University of Missouri, Columbia, MO, USA. Electronic address: rohlfingc@missouri.edu., Hanson S; Department of Pathology and Anatomical Sciences, University of Missouri, Columbia, MO, USA., Estey MP; DynaLIFE Medical Labs, Edmonton, Alberta, Canada., Bordeleau P; DynaLIFE Medical Labs, Edmonton, Alberta, Canada., Little RR; Department of Pathology and Anatomical Sciences, University of Missouri, Columbia, MO, USA.
Jazyk: angličtina
Zdroj: Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2021 Nov; Vol. 522, pp. 31-35. Date of Electronic Publication: 2021 Aug 02.
DOI: 10.1016/j.cca.2021.07.027
Abstrakt: Background: Hemoglobin C, D Punjab, E or S trait can interfere with hemoglobin A1c (HbA1c) results. We assessed whether they affect results obtained with 15 current assay methods.
Methods: Hemoglobin AA (HbAA), HbAC, HbAD Punjab, HbAE and HbAS samples were analyzed on 2 enzymatic, 4 ion-exchange HPLC and 9 immunoassay methods. Trinity Premier Hb9210 boronate affinity HPLC was the comparative method. An overall test of coincidence of least-squared linear regression lines was performed to determine if HbA1c results were statistically significantly different from those of HbAA samples. Clinically significant interference was defined as >6% difference from HbAA at 6 or 9% HbA1c compared to Premier Hb9210 using Deming regression.
Results: All methods showed statistically significant effects for one or more variants. Clinically significant effects were observed for the Tosoh G11 variant mode (HbAD), Roche b 101 (HbAC and HbAE) and Siemens DCA Vantage (HbAE and HbAS). All other methods (Beckman Coulter B93009 and B00389 on DxC700AU, and Unicel DxC, Ortho Clinical Vitros 5.1, Roche cobas c 513, Siemens Dimension RxL and Vista, and Enzymatic on Advia and Atellica, Tosoh G8 5.24 and 5.28, and GX) showed no clinically significant differences.
Conclusions: A few methods showed interference from one or more variants. Laboratories need to be aware of potential HbA1c assay interferences.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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