LINC00665 knockdown protects against cerebral ischemia-reperfusion injury.

Autor:	Yan TS; Department of Critical Care Medicine, Zhangqiu District People's Hospital, Jinan, China., Ma CH; Clinical Skills Training Center, Qingdao Municipal Hospital, Qingdao, China., Peng N; Operation Room, Zhangqiu District People's Hospital, Jinan, China., Li YE; Department of Medicine, Zhangqiu District People's Hospital, Jinan, China., Li QY; Image Center, Zhangqiu District People's Hospital, Jinan, China., Wang H; Department of Anesthesiology, The Third People's Hospital of Qingdao, Qingdao, China.
Jazyk:	angličtina
Zdroj:	Journal of biological regulators and homeostatic agents [J Biol Regul Homeost Agents] 2021 Jul 30; Vol. 35 (Spec Issue on Internal Medicine n.1). Date of Electronic Publication: 2021 Jul 30.
DOI:	10.23812/21-SI1-8
Abstrakt:	LINC00665 has been reported to participate in several human diseases. However, the role of LINC00665 in cerebral ischemia-reperfusion (CI/R) is still unknown. This study is designed to investigate the role of LINC00665 in rats with CI/R injury. We established middle cerebral artery occlusion/ reperfusion (MCAO/R) rats model in vivo. PC12 cells treated with oxygen-glucose deprivation/reperfusion (OGD/R) were used to establish in vitro I/R model. RT-qPCR assay was adopted to assess the mRNA expression of LINC00665 and miR-744-5p. MTT assay was used to determine cell viability. The protein expression of Bax and Bcl-2 were detected by Western blot assay. The relationship between LINC00665 and miR-744-5p was confirmed by dual luciferase reporter assay and RNA immunoprecipitation (RIP). In this study, we found that LINC00665 was sharply up regulated in MCAO/R rats and PC12 cells treated with I/R. Functionally, LINC00665 knockdown attenuated oxidative damage in PC12 cells treated with I/R. Moreover, LINC00665 knockdown promoted cell viability, while inhibited cell apoptosis in PC12 cells treated with I/R. In addition, miR-744-5p was confirmed to be a target of LINC00665. LINC00665 knockdown was validated to project CI/R injury by sponging miR-744-5p expression. (Copyright 2020 Biolife Sas. www.biolifesas.org.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu