Larval excretion/secretion of dipters of Lucilia cuprina species induces death in promastigote and amastigote forms of Leishmania amazonensis.

Autor:	Rodrigues ACJ; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, Center for Biological Sciences, State University of Londrina-UELRod. Celso Garcia Cid, Zip code 86057-970, Londrina, PR, Brazil., Bortoleti BTDS; Biosciences and Biotechnology Postgraduate Program, Carlos Chagas Institute, (ICC/Fiocruz/PR), Professor Algacyr Munhoz Mader, Zip code 81350-010, Curitiba, PR, Brazil., Carloto ACM; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, Center for Biological Sciences, State University of Londrina-UELRod. Celso Garcia Cid, Zip code 86057-970, Londrina, PR, Brazil., Silva TF; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, Center for Biological Sciences, State University of Londrina-UELRod. Celso Garcia Cid, Zip code 86057-970, Londrina, PR, Brazil., Concato VM; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, Center for Biological Sciences, State University of Londrina-UELRod. Celso Garcia Cid, Zip code 86057-970, Londrina, PR, Brazil., Gonçalves MD; Biotransformation and Phytochemistry Laboratory, Department of Chemistry, Center for Exact Sciences, State University of Londrina, Rod. Celso Garcia Cid, Zip code 86057-970 PR, Brazil., Tomiotto-Pelissier F; Biosciences and Biotechnology Postgraduate Program, Carlos Chagas Institute, (ICC/Fiocruz/PR), Professor Algacyr Munhoz Mader, Zip code 81350-010, Curitiba, PR, Brazil., Detoni MB; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, Center for Biological Sciences, State University of Londrina-UELRod. Celso Garcia Cid, Zip code 86057-970, Londrina, PR, Brazil., Diaz-Roa A; Special Laboratory for Applied Toxicology, Instituto Butantan, Vital Brasil Avenue, Zip code 055503-900, São Paulo, SP, Brazil., Júnior PIDS; Special Laboratory for Applied Toxicology, Instituto Butantan, Vital Brasil Avenue, Zip code 055503-900, São Paulo, SP, Brazil., Costa IN; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, Center for Biological Sciences, State University of Londrina-UELRod. Celso Garcia Cid, Zip code 86057-970, Londrina, PR, Brazil., Conchon-Costa I; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, Center for Biological Sciences, State University of Londrina-UELRod. Celso Garcia Cid, Zip code 86057-970, Londrina, PR, Brazil., Bidoia DL; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, Center for Biological Sciences, State University of Londrina-UELRod. Celso Garcia Cid, Zip code 86057-970, Londrina, PR, Brazil., Miranda-Sapla MM; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, Center for Biological Sciences, State University of Londrina-UELRod. Celso Garcia Cid, Zip code 86057-970, Londrina, PR, Brazil., Pavanelli WR; Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, Center for Biological Sciences, State University of Londrina-UELRod. Celso Garcia Cid, Zip code 86057-970, Londrina, PR, Brazil.
Jazyk:	angličtina
Zdroj:	Pathogens and disease [Pathog Dis] 2021 Aug 10; Vol. 79 (6).
DOI:	10.1093/femspd/ftab040
Abstrakt:	Leishmaniasis is a neglected tropical disease that affects millions of people around the world. Larval excretion/secretion (ES) of the larvae of flies of the Calliphoridae family has microbicidal activity against Gram-positive and Gram-negative bacteria, in addition to some species of Leishmania. Our study aimed at assessing the in vitro efficacy of Lucilia cuprina larval ES against the promastigote and amastigote forms of Leishmania amazonensis, elucidating possible microbicidal mechanisms and routes of death involved. Larval ES was able to inhibit the viability of L. amazonensis at all concentrations, induce morphological and ultrastructural changes in the parasite, retraction of the cell body, roughness of the cytoplasmic membrane, leakage of intracellular content, ROS production increase, induction of membrane depolarization and mitochondrial swelling, the formation of cytoplasmic lipid droplets and phosphatidylserine exposure, thus indicating the possibility of apoptosis-like death. To verify the efficacy of larval ES on amastigote forms, we performed a phagocytic assay, measurement of total ROS and NO. Treatment using larval ES reduced the percentage of infection and the number of amastigotes per macrophage of lineage J774A.1 at all concentrations, increasing the production of ROS and TNF-α, thus indicating possible pro-inflammatory immunomodulation and oxidative damage. Therefore, treatment using larval ES is effective at inducing the death of promastigotes and amastigotes of L. amazonensis even at low concentrations. (© The Author(s) 2021. Published by Oxford University Press on behalf of FEMS. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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