Retrospective cell lineage reconstruction in humans by using short tandem repeats.

Autor:	Tao L; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 761001, Israel., Raz O; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 761001, Israel., Marx Z; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 761001, Israel., Ghosh MS; Experimental Medicine and Therapy Research, University of Regensburg, Franz-Josef-Strauß-Allee 11, 93053 Regensburg, Germany., Huber S; Experimental Medicine and Therapy Research, University of Regensburg, Franz-Josef-Strauß-Allee 11, 93053 Regensburg, Germany., Greindl-Junghans J; Experimental Medicine and Therapy Research, University of Regensburg, Franz-Josef-Strauß-Allee 11, 93053 Regensburg, Germany., Biezuner T; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 761001, Israel., Amir S; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 761001, Israel., Milo L; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 761001, Israel., Adar R; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 761001, Israel., Levy R; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 761001, Israel., Onn A; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 761001, Israel., Chapal-Ilani N; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 761001, Israel., Berman V; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 761001, Israel., Ben Arie A; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 761001, Israel., Rom G; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 761001, Israel., Oron B; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 761001, Israel., Halaban R; Department of Dermatology, Yale University School of Medicine, New Haven, CT 06520-8059, USA., Czyz ZT; Experimental Medicine and Therapy Research, University of Regensburg, Franz-Josef-Strauß-Allee 11, 93053 Regensburg, Germany., Werner-Klein M; Experimental Medicine and Therapy Research, University of Regensburg, Franz-Josef-Strauß-Allee 11, 93053 Regensburg, Germany., Klein CA; Experimental Medicine and Therapy Research, University of Regensburg, Franz-Josef-Strauß-Allee 11, 93053 Regensburg, Germany.; Division of Personalized Tumor Therapy, Fraunhofer Institute for Experimental Medicine and Toxicology Regensburg, Am Biopark 9, 93053 Regensburg, Germany., Shapiro E; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 761001, Israel.
Jazyk:	angličtina
Zdroj:	Cell reports methods [Cell Rep Methods] 2021 Jul 26; Vol. 1 (3), pp. None. Date of Electronic Publication: 2021 Jul 26.
DOI:	10.1016/j.crmeth.2021.100054
Abstrakt:	Cell lineage analysis aims to uncover the developmental history of an organism back to its cell of origin. Recently, novel in vivo methods utilizing genome editing enabled important insights into the cell lineages of animals. In contrast, human cell lineage remains restricted to retrospective approaches, which still lack resolution and cost-efficient solutions. Here, we demonstrate a scalable platform based on short tandem repeats targeted by duplex molecular inversion probes. With this human cell lineage tracing method, we accurately reproduced a known lineage of DU145 cells and reconstructed lineages of healthy and metastatic single cells from a melanoma patient who matched the anatomical reference while adding further refinements. This platform allowed us to faithfully recapitulate lineages of developmental tissue formation in healthy cells. In summary, our lineage discovery platform can profile informative somatic mutations efficiently and provides solid lineage reconstructions even in challenging low-mutation-rate healthy single cells. Competing Interests: The authors declare no competing financial interests. (© 2021 The Authors.)
Databáze:	MEDLINE
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