Quantifying nanodiamonds biodistribution in whole cells with correlative iono-nanoscopy.

Autor: Mi Z; Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore, Singapore., Chen CB; Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore, Singapore., Tan HQ; Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore, Singapore.; Division of Radiation Oncology, National Cancer Centre Singapore, Singapore, Singapore., Dou Y; Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore, Singapore., Yang C; Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore, Singapore., Turaga SP; Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore, Singapore., Ren M; Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore, Singapore., Vajandar SK; Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore, Singapore., Yuen GH; Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore, Singapore., Osipowicz T; Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore, Singapore., Watt F; Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore, Singapore. phywattf@nus.edu.sg., Bettiol AA; Centre for Ion Beam Applications, Department of Physics, National University of Singapore, Singapore, Singapore. a.bettiol@nus.edu.sg.; Division of Science, Yale-NUS College, Singapore, Singapore. a.bettiol@nus.edu.sg.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2021 Aug 02; Vol. 12 (1), pp. 4657. Date of Electronic Publication: 2021 Aug 02.
DOI: 10.1038/s41467-021-25004-9
Abstrakt: Correlative imaging and quantification of intracellular nanoparticles with the underlying ultrastructure is crucial for understanding cell-nanoparticle interactions in biological research. However, correlative nanoscale imaging of whole cells still remains a daunting challenge. Here, we report a straightforward nanoscopic approach for whole-cell correlative imaging, by simultaneous ionoluminescence and ultrastructure mapping implemented with a highly focused beam of alpha particles. We demonstrate that fluorescent nanodiamonds exhibit fast, ultrabright and stable emission upon excitation by alpha particles. Thus, by using fluorescent nanodiamonds as imaging probes, our approach enables quantification and correlative localization of single nanodiamonds within a whole cell at sub-30 nm resolution. As an application example, we show that our approach, together with Monte Carlo simulations and radiobiological experiments, can be employed to provide unique insights into the mechanisms of nanodiamond radiosensitization at the single whole-cell level. These findings may benefit clinical studies of radio-enhancement effects by nanoparticles in charged-particle cancer therapy.
(© 2021. The Author(s).)
Databáze: MEDLINE
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