DNA barcoding of brackish and marine water fishes and shellfishes of Sundarbans, the world's largest mangrove ecosystem.

Autor: Habib KA; Faculty of Fisheries, Department of Fisheries Biology and Genetics, Aquaculture and Marine Science, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.; Department of Fisheries Biology and Genetics, Aquatic Bioresource Research Lab, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh., Neogi AK; Department of Fisheries Biology and Genetics, Aquatic Bioresource Research Lab, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh., Rahman M; Department of Biological Science, Wayne State University, Detroit, Michigan, United States of America., Oh J; Marine Biology and Biological Oceanography Division, Korea Institute of Ocean Science and Technology (KIOST), Busan, Korea., Lee YH; Marine Biology and Biological Oceanography Division, Korea Institute of Ocean Science and Technology (KIOST), Busan, Korea., Kim CG; Marine Biology and Biological Oceanography Division, Korea Institute of Ocean Science and Technology (KIOST), Busan, Korea.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2021 Aug 02; Vol. 16 (8), pp. e0255110. Date of Electronic Publication: 2021 Aug 02 (Print Publication: 2021).
DOI: 10.1371/journal.pone.0255110
Abstrakt: The present study aims to apply a DNA barcoding tool through amplifying two mitochondrial candidate genes i.e., COI and 16S rRNA for accurate identification of fish, aquatic molluscs and crustaceans of Sundarbans mangrove wetland, to build a reference library of fish and shellfishes of this unique ecosystems. A total of 185 mitochondrial COI barcode sequences and 59 partial sequences of the 16S rRNA gene were obtained from 120 genera, 65 families and 21 orders of fish, crustaceans and molluscs. The collected samples were first identified by examining morphometric characteristics and then assessed by DNA barcoding. The COI and 16S rRNA sequences of fishes and crustaceans were clearly discriminated among genera in their phylogenies. The average Kimura two-parameter (K2P) distances of COI barcode sequences within species, genera, and families of fishes are 1.57±0.06%, 15.16±0.23%, and 17.79±0.02%, respectively, and for 16S rRNA sequences, these values are 1.74±.8%, 0.97±.8%, and 4.29±1.3%, respectively. The minimum and maximum K2P distance based divergences in COI sequences of fishes are 0.19% and 36.27%, respectively. In crustaceans, the K2P distances within genera, families, and orders are 1.4±0.03%, 17.73±0.15%, and 22.81±0.02%, respectively and the minimum and maximum divergences are 0.2% and 33.93%, respectively. Additionally, the present study resolves the misidentification of the mud crab species of the Sundarbans as Scylla olivacea which was previously stated as Scylla serrata. In case of molluscs, values of interspecific divergence ranges from 17.43% to 66.3% in the barcoded species. The present study describes the development of a molecular and morphometric cross-referenced inventory of fish and shellfish of the Sundarbans. This inventory will be useful in future biodiversity studies and in forming future conservation plan.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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