The dorsal tergite cuticle of Helleria brevicornis: Ultrastructure, mineral distribution, calcite microstructure and texture.
| Autor: | Seidl B; Central Facility for Electron Microscopy, University of Ulm, Albert-Einstein-Allee 11, 89069 Ulm, Germany., Reisecker C; Department of Polymer Science, Johannes Kepler University Linz, Altenbergerstraße 69, 4040 Linz, Austria., Neues F; Institute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitätsstrasse 5-7, 45117Essen, Germany., Campanaro A; Council for Agricultural Research and Economics, Research Centre for Plant and Certification, Via di Lanciola 12/a, I-50125 Cascine del Riccio, Florence, Italy., Epple M; Institute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitätsstrasse 5-7, 45117Essen, Germany., Hild S; Department of Polymer Science, Johannes Kepler University Linz, Altenbergerstraße 69, 4040 Linz, Austria., Ziegler A; Central Facility for Electron Microscopy, University of Ulm, Albert-Einstein-Allee 11, 89069 Ulm, Germany. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of structural biology: X [J Struct Biol X] 2021 Jul 10; Vol. 5, pp. 100051. Date of Electronic Publication: 2021 Jul 10 (Print Publication: 2021). |
| DOI: | 10.1016/j.yjsbx.2021.100051 |
| Abstrakt: | Among the terrestrial Crustacea, isopods have most successfully established themselves in a large variety of terrestrial habitats. As in most Crustacea, their cuticle consists of a hierarchically organised organic phase of chitin-protein fibrils, containing calcium carbonate and some calcium phosphate. In previous studies, we examined the tergite cuticle of Tylos europaeus, which lives on seashores and burrows into moist sand. In this study, we investigate the closely related species Helleria brevicornis, which is completely terrestrial and lives in leaf litter and humus and burrows into the soil. To get deeper insights in relation between the structure of the organic and mineral phase in species living in diverse habitats, we have investigated the structure, and the chemical and crystallographic properties of the tergite cuticle using various preparation techniques, and microscopic and analytical methods. The results reveal long and short epicuticular sensilla with brushed tips on the tergite surface that do not occur in T. europaeus. As in T. europaeus a distal exocuticle, which contains a low number of organic fibres, contains calcite while the subjacent layers of the exo- and endocuticle contain amorphous calcium carbonate. The distal exocuticle contains a polygonal pattern of mineral initiation sites that correspond to interprismatic septa described for decapod crabs. The shape and position of calcite units do not follow the polygonal pattern of the septa. The results indicate that the calcite units form by crystallisation from an amorphous phase that progresses from both margins of the septa to the centres of the polygons. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2021 The Authors. Published by Elsevier Inc.) |
| Databáze: | MEDLINE |
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