MAPK and Notch-Mediated Effects of Meso-Xanthin F199 Compounds on Proliferative Activity and Apoptosis of Human Melanocytes in Three-Dimensional Culture.

Autor: Saburina IN; Institute of General Pathology and Pathophysiology, 8 Baltiyskaya Str., 125315 Moscow, Russia., Zurina IM; Institute of General Pathology and Pathophysiology, 8 Baltiyskaya Str., 125315 Moscow, Russia.; Institute for Regenerative Medicine, Sechenov First Moscow State Medical University, 8-2 Trubetskaya Str., 119991 Moscow, Russia., Kosheleva NV; Institute of General Pathology and Pathophysiology, 8 Baltiyskaya Str., 125315 Moscow, Russia.; Institute for Regenerative Medicine, Sechenov First Moscow State Medical University, 8-2 Trubetskaya Str., 119991 Moscow, Russia.; World-Class Research Center 'Digital Biodesign and Personalized Healthcare', Sechenov First Moscow State Medical University, 8-2 Trubetskaya Str., Moscow 119991, Russia., Gorkun AA; Institute of General Pathology and Pathophysiology, 8 Baltiyskaya Str., 125315 Moscow, Russia.; Institute for Regenerative Medicine, Sechenov First Moscow State Medical University, 8-2 Trubetskaya Str., 119991 Moscow, Russia., Volkova EN; Institute of General Pathology and Pathophysiology, 8 Baltiyskaya Str., 125315 Moscow, Russia., Grinakovskaya OS; Institute of General Pathology and Pathophysiology, 8 Baltiyskaya Str., 125315 Moscow, Russia., Rybakov AS; Institute of General Pathology and Pathophysiology, 8 Baltiyskaya Str., 125315 Moscow, Russia., Kaysheva AL; Institute of Biomedical Chemistry, Biobanking Group, 10 Pogodinskaya Str., Bld. 8, 119121 Moscow, Russia., Kopylov AT; Institute of Biomedical Chemistry, Biobanking Group, 10 Pogodinskaya Str., Bld. 8, 119121 Moscow, Russia., Morozov SG; Institute of General Pathology and Pathophysiology, 8 Baltiyskaya Str., 125315 Moscow, Russia.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2021 Jul 20; Vol. 2021, pp. 8463161. Date of Electronic Publication: 2021 Jul 20 (Print Publication: 2021).
DOI: 10.1155/2021/8463161
Abstrakt: Meso-Xanthin (Meso-Xanthin F199™) is a highly active antiaging injection drug of the latest generation. The main acting compound is fucoxanthin, supplemented with several growth factors, vitamins, and hyaluronic acid. Previous examination of fucoxanthin on melanocytes showed its ability to inhibit skin pigmentation through different signaling pathways focused on suppression of melanogenic-stimulating receptors. In turn, the anticancer property of fucoxanthin is realized through MAPK and PI3K pathways. We aimed to evaluate the effect of fucoxanthin and supplemented growth factors on melanocyte growth and transformation at a proteomic level. The effect of fucoxanthin on melanocytes cultivated in three-dimensional (3D) condition was examined using high-throughput proteomic and system biology approaches to disclose key molecular events of the targeted action. Our results demonstrated significant inhibition of cell differentiation and ubiquitination processes. We found that the negative regulation of PSME1 and PTGIS largely determines the inhibition of NF- κ B and MAPK2. Besides, fucoxanthin selectively inhibits cell differentiation via negative regulation of Raf signaling and the upstream activation of IL-1 signaling. It is assumed that inhibition of Raf influences the Notch-4 signaling and switches off the MAPK/MAPK2 cascade. Blockage of MAPK/MAPK2 is feasible due to suppression of Ras and NF- κ B by the addressed action of IKKB , IKK2 , and TRAF6 . Suggestively, Meso-Xanthin F199™ can manage processes of proliferative activity and inhibition of apoptosis due to composition of fucoxanthin and growth-stimulating factors, which may increase the risk of skin cancer development under certain condition.
Competing Interests: The authors declared no conflict of interests.
(Copyright © 2021 Irina N. Saburina et al.)
Databáze: MEDLINE
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