Long intergenic non-coding RNA, regulator of reprogramming (LINC-ROR) over-expression predicts poor prognosis in renal cell carcinoma.

Autor:	Fawzy MS; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.; Department of Biochemistry, Faculty of Medicine, Northern Border University, Arar, Saudi Arabia., Toraih EA; Genetics Unit, Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.; Center of Excellence of Molecular and Cellular Medicine, Suez Canal University, Ismailia, Egypt., El-Wazir A; Genetics Unit, Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.; Center of Excellence of Molecular and Cellular Medicine, Suez Canal University, Ismailia, Egypt., Hosny MM; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt., Badran DI; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt., El Kelish A; Botany Department, Faculty of Science, Suez Canal University, Ismailia, Egypt.
Jazyk:	angličtina
Zdroj:	Archives of medical science : AMS [Arch Med Sci] 2019 May 17; Vol. 17 (4), pp. 1016-1027. Date of Electronic Publication: 2019 May 17 (Print Publication: 2021).
DOI:	10.5114/aoms.2019.85201
Abstrakt:	Introduction: Long intergenic non-coding RNA, regulator of reprogramming (LINC-ROR) is a newly identified cytoplasmic long non-coding RNA (lncRNA) implicated in cell longevity and apoptosis. We aimed in the current work for the first time to investigate the association of the expression profiles of LINC-ROR and three stem-related transcriptional factors with clinicopathological data and their impact on renal cell carcinoma (RCC) progression in a sample of RCC patients. Material and Methods: Expression levels of LINC-ROR and stemness-related factors: SOX2, NANOG, and POU5F1 were detected in 60 formalin-fixed, paraffin-embedded tissues, and their paired adjacent non-cancer tissues ( n = 60) by using real-time qRT-PCR analysis. Additionally, the expression profiles were compared with the available clinicopathological features. Results: The genes studied were markedly up-regulated in RCC (medians and interquartile ranges were 30.3 (1.84-235.5), 10.2 (1.84-53.9), 5.39 (0.94-23.5), and 12.5 (1.61-43.2) for LINC-ROR , SOX2 , NANOG , and POU5F1 , respectively) relative to paired non-cancer tissue. High expression levels were associated with poor prognosis in terms of tumour undifferentiation (for LINC-ROR, SOX2 , and NANOG ), lymph node infiltration (for SOX2 ), postoperative recurrence (for LINC-ROR and SOX2 ), and shorter overall survival (OS) and progression-free survival (for all genes studied). The best curve for OS prediction was constructed with LINC-ROR data (area under the receiver operating characteristic curve (AUC) = 0.804 at a cut-off value of 72.7, sensitivity 78.9%, and specificity 80.5%). Conclusions: Collectively, aberrant LINC-ROR and pluripotent gene expression may be recognised as prognostic markers for RCC. Future functional studies are highly recommended to validate the study findings. Competing Interests: The authors declare no conflict of interest. (Copyright: © 2019 Termedia & Banach.)
Databáze:	MEDLINE
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