Memory B cell repertoire for recognition of evolving SARS-CoV-2 spike.

Autor:	Tong P; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA., Gautam A; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA., Windsor IW; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA; Laboratory of Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA., Travers M; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA., Chen Y; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA., Garcia N; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA., Whiteman NB; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA., McKay LGA; Department of Microbiology, Boston University School of Medicine, Boston, MA 02115, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA 02115, USA., Storm N; Department of Microbiology, Boston University School of Medicine, Boston, MA 02115, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA 02115, USA., Malsick LE; Department of Microbiology, Boston University School of Medicine, Boston, MA 02115, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA 02115, USA., Honko AN; Department of Microbiology, Boston University School of Medicine, Boston, MA 02115, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA 02115, USA., Lelis FJN; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA., Habibi S; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA., Jenni S; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA., Cai Y; Laboratory of Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA., Rennick LJ; The Center for Vaccine Research, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA; The Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Duprex WP; The Center for Vaccine Research, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA; The Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., McCarthy KR; The Center for Vaccine Research, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA; The Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Lavine CL; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA., Zuo T; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA., Lin J; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA., Zuiani A; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA., Feldman J; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA., MacDonald EA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA., Hauser BM; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA., Griffths A; Department of Microbiology, Boston University School of Medicine, Boston, MA 02115, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA 02115, USA., Seaman MS; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA., Schmidt AG; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA; Department of Microbiology, Harvard Medical School, Boston, MA 02115, USA; Massachusetts Consortium on Pathogen Readiness, Boston, MA 02115, USA., Chen B; Laboratory of Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA; Massachusetts Consortium on Pathogen Readiness, Boston, MA 02115, USA., Neuberg D; Department of Data Science, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Bajic G; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA; Laboratory of Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA., Harrison SC; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA; Laboratory of Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA; Massachusetts Consortium on Pathogen Readiness, Boston, MA 02115, USA; Howard Hughes Medical Institute, Boston, MA 02115, USA., Wesemann DR; Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Massachusetts Consortium on Pathogen Readiness, Boston, MA 02115, USA. Electronic address: dwesemann@bwh.harvard.edu.
Jazyk:	angličtina
Zdroj:	Cell [Cell] 2021 Sep 16; Vol. 184 (19), pp. 4969-4980.e15. Date of Electronic Publication: 2021 Jul 23.
DOI:	10.1016/j.cell.2021.07.025
Abstrakt:	Memory B cell reserves can generate protective antibodies against repeated SARS-CoV-2 infections, but with unknown reach from original infection to antigenically drifted variants. We charted memory B cell receptor-encoded antibodies from 19 COVID-19 convalescent subjects against SARS-CoV-2 spike (S) and found seven major antibody competition groups against epitopes recurrently targeted across individuals. Inclusion of published and newly determined structures of antibody-S complexes identified corresponding epitopic regions. Group assignment correlated with cross-CoV-reactivity breadth, neutralization potency, and convergent antibody signatures. Although emerging SARS-CoV-2 variants of concern escaped binding by many members of the groups associated with the most potent neutralizing activity, some antibodies in each of those groups retained affinity-suggesting that otherwise redundant components of a primary immune response are important for durable protection from evolving pathogens. Our results furnish a global atlas of S-specific memory B cell repertoires and illustrate properties driving viral escape and conferring robustness against emerging variants. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu