Nile tilapia skin (Oreochromis niloticus) for burn treatment: ultrastructural analysis and quantitative assessment of collagen.

Autor: Lima Verde MEQ; Post Graduate Program in Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil; Drugs Research and Development Center, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: elisalimaverde@gmail.com., Ferreira-Júnior AEC; Post Graduate Program in Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil; Drugs Research and Development Center, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: ernando-junior@hotmail.com., de Barros-Silva PG; Post Graduate Program in Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil; Drugs Research and Development Center, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: paulo_goberlanio@yahoo.com.br., Miguel EC; Analytical Center, Physics Department, Science Center, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: emilio@centralanalitica.ufc.br., Mathor MB; Nuclear and Energy Research Institute, IPEN-CNEN/SP, São Paulo, São Paulo, Brazil. Electronic address: mathor@tcoop.coop.br., Lima-Júnior EM; Burn Treatment Center, Dr José Frota Institute (IJF), Fortaleza, Ceará, Brazil. Electronic address: edmarmaciel@gmail.com., de Moraes-Filho MO; Drugs Research and Development Center, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: odorico@ufc.br., Alves APNN; Post Graduate Program in Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil; Drugs Research and Development Center, Federal University of Ceará, Fortaleza, Ceará, Brazil. Electronic address: ananegreirosnunes@gmail.com.
Jazyk: angličtina
Zdroj: Acta histochemica [Acta Histochem] 2021 Sep; Vol. 123 (6), pp. 151762. Date of Electronic Publication: 2021 Jul 29.
DOI: 10.1016/j.acthis.2021.151762
Abstrakt: Nile tilapia (Oreochromis niloticus) skin is a well-known biomaterial used as an occlusive dressing for burn treatment. It is also an inexpensive and important source of collagen. This study aims to describe the ultrastructural aspects of Nile tilapia skin, assess its collagen amount and organization, and compare quantitative methods of histochemical and immunohistochemical analysis (in all sterilization steps for use in burn dressings). One sample (0.5 × 0.5 cm) of ten different fish skins was divided in four groups: in natura skin (IN), chemical sterilization (CH), additional irradiation (30 kGy) (IR), and skins used in burn treatment (BT) to compare histochemical and immunohistochemical findings of collagen amount and describe ultrastructural aspects through scanning electron microscopy. The amount of type I collagen decreased during sterilization and clinical use owing to gradual reduction of immunostaining (anti-collagen-I) and decreasing fiber thickness of the collagen, when compared to type III (Picrosirius-red-polarized light). The collagen fibers were rearranged at each sterilization step, with a low collagen percentage and large structural disorganization in BT. The amount of type-I collagen was further reduced after BT (p < 0.05). Both the methods did not exhibit a quantified value difference (p = 0.247), and a positive correlation (r = 0.927; 95 % CI = 0.720-0.983) was observed between them, with concordance for collagen quantification in similar samples, presenting a low systematic error rate (Dalberg coefficient: 6.70). A significant amount of type-I collagen is still observed despite sterilization, although clinical application further reduces type I collagen. Its quantification can be performed both by immunohistochemistry and/or Picrosirius Red reliably.
(Copyright © 2021 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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