EBV downregulates the m 6 A "writer" WTAP in EBV-associated gastric carcinoma.

Autor: Xiao H; Department of Medical microbiology, School of Basic Medicine, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China., Zhang Y; Department of Clinical Laboratory, Central Hospital of Zibo, 19 Gongqingtuan Road, Zibo, 255036, China., Sun L; Department of Pathology, Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China., Zhao Z; Department of Central Laboratory, Qingdao Municipal Hospital, Qingdao, 266000, China., Liu W; Department of Medical microbiology, School of Basic Medicine, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China., Luo B; Department of Medical microbiology, School of Basic Medicine, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China. Electronic address: qdluobing@163.com.
Jazyk: angličtina
Zdroj: Virus research [Virus Res] 2021 Oct 15; Vol. 304, pp. 198510. Date of Electronic Publication: 2021 Jul 28.
DOI: 10.1016/j.virusres.2021.198510
Abstrakt: Epstein-Barr virus-associated gastric carcinoma (EBVaGC) is characterized by the clonal growth of EBV-infected stomach epithelial cells. It has been reported that N 6 -methyladenosine (m 6 A) methylation can regulate the splicing, expression, decay and translation of mRNAs. Wilms' tumor 1-associating protein (WTAP) is an m 6 A "writer" with methyltransferase activity. An m 6 A RNA methylation quantification kit and immunofluorescence (IF) showed that the m 6 A total RNA methylation level of the Epstein-Barr virus-negative gastric carcinoma (EBVnGC) cell line (SGC7901) was higher than that in the EBVaGC cell line (GT38). To investigate the underlying mechanism of the downregulated expression of m 6 A RNA methylation, we analyzed the expression of WTAP. The results showed that the expression of WTAP protein in EBVaGC cell lines was significantly lower than that in EBVnGC cell lines according to western blotting and IF. Through plasmid overexpression and RNA interference technology, we further found that EBV-encoded small RNA1 (EBER1) could downregulate WTAP expression by activating the NF-κB signaling pathway. In addition, WTAP could increase proliferation and inhibit migration in gastric carcinoma cell lines. In summary, EBER1 of EBV potentially regulated WTAP by affecting the NF-κB signaling pathway and WTAP further affected cell proliferation and migration.
(Copyright © 2021. Published by Elsevier B.V.)
Databáze: MEDLINE
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