Long-term exposure of Daphnia magna to polystyrene microplastic (PS-MP) leads to alterations of the proteome, morphology and life-history.

Autor: Trotter B; University of Bayreuth, Animal Ecology 1, Universitätsstraße 30, 95447 Bayreuth, Germany; Gene Center Munich, Laboratory for Functional Genome Analysis (LAFUGA), LMU München, Feodor-Lynen Straße 25, 81377 Munich, Germany., Wilde MV; Gene Center Munich, Laboratory for Functional Genome Analysis (LAFUGA), LMU München, Feodor-Lynen Straße 25, 81377 Munich, Germany. Electronic address: wilde@genzentrum.lmu.de., Brehm J; University of Bayreuth, Animal Ecology 1, Universitätsstraße 30, 95447 Bayreuth, Germany. Electronic address: Julian1.Brehm@uni-bayreuth.de., Dafni E; University of Bayreuth, Animal Ecology 1, Universitätsstraße 30, 95447 Bayreuth, Germany., Aliu A; Gene Center Munich, Laboratory for Functional Genome Analysis (LAFUGA), LMU München, Feodor-Lynen Straße 25, 81377 Munich, Germany. Electronic address: Arlinda.Aliu@campus.lmu.de., Arnold GJ; Gene Center Munich, Laboratory for Functional Genome Analysis (LAFUGA), LMU München, Feodor-Lynen Straße 25, 81377 Munich, Germany. Electronic address: Arnold@genzentrum.lmu.de., Fröhlich T; Gene Center Munich, Laboratory for Functional Genome Analysis (LAFUGA), LMU München, Feodor-Lynen Straße 25, 81377 Munich, Germany. Electronic address: Frohlich@genzentrum.lmu.de., Laforsch C; University of Bayreuth, Animal Ecology 1, Universitätsstraße 30, 95447 Bayreuth, Germany. Electronic address: Christian.Laforsch@uni-bayreuth.de.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2021 Nov 15; Vol. 795, pp. 148822. Date of Electronic Publication: 2021 Jul 03.
DOI: 10.1016/j.scitotenv.2021.148822
Abstrakt: In the past years, the research focus on the effects of MP on aquatic organisms extended from marine systems towards freshwater systems. An important freshwater model organism in the MP field is the cladoceran Daphnia, which plays a central role in lacustrine ecosystems and has been established as a test organism in ecotoxicology. To investigate the effects of MP on Daphnia magna, we performed a chronic exposure experiment with polystyrene MP under strictly standardized conditions. Chronic exposure of D. magna to PS microparticles led to a significant reduction in body length and number of offspring. To shed light on underlying molecular mechanisms induced by microplastic ingestion in D. magna, we assessed the effects of PS-MP at the proteomic level, as proteins, e.g., enzymes, are especially relevant for an organism's physiology. Using a state-of-the-art mass spectrometry based approach, we were able to identify 28,696 different peptides, which could be assigned to 3784 different proteins. Using a customized bioinformatic workflow, we identified 41 proteins significantly altered in abundance (q-value <0.05) in the PS exposed D. magna. Among the proteins increased in the PS treated group were several sulfotransferases, involved in basic biochemical pathways, as well as GABA transaminase catalyzing the degradation of the neurotransmitter GABA. In the abundance decreased group, we found essential proteins such as the DNA-directed RNA polymerase subunit and other proteins connected to biotic and inorganic stress and reproduction. Strikingly, we further identified several digestive enzymes that are significantly downregulated in the PS treated animals, which could have interfered with the affected animal's nutrient supply. This may explain the altered morphological and life history traits of the PS exposed daphnids. Our results indicate that long-term exposure to PS microplastics, which are frequently detected in environmental samples, may affect the fitness of daphnids.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: