| Autor: |
Gheorghe CE; Department of Psychiatry and Neurobehavioral Science, University College Cork, Cork, Ireland.; Department of Anatomy and Neuroscience, University College Cork, Cork, Ireland.; APC Microbiome Ireland, University College Cork, Cork, Ireland., Ritz NL; Department of Anatomy and Neuroscience, University College Cork, Cork, Ireland.; APC Microbiome Ireland, University College Cork, Cork, Ireland., Martin JA; Department of Psychiatry and Neurobehavioral Science, University College Cork, Cork, Ireland.; APC Microbiome Ireland, University College Cork, Cork, Ireland., Wardill HR; Precision Medicine, South Australian Health and Medical Research Institute (SAHMRI), Adelaide, Australia.; Adelaide Medical School, the University of Adelaide, Adelaide, Australia., Cryan JF; Department of Psychiatry and Neurobehavioral Science, University College Cork, Cork, Ireland.; Department of Anatomy and Neuroscience, University College Cork, Cork, Ireland.; APC Microbiome Ireland, University College Cork, Cork, Ireland., Clarke G; Department of Psychiatry and Neurobehavioral Science, University College Cork, Cork, Ireland.; APC Microbiome Ireland, University College Cork, Cork, Ireland.; INFANT Research Centre, University College Cork, Cork, Ireland. |
| Abstrakt: |
In recent years, studies investigating the role of the gut microbiota in health and diseases have increased enormously - making it essential to deepen and question the research methodology employed. Fecal microbiota transplantation (FMT) in rodent studies (either from human or animal donors) allows us to better understand the causal role of the intestinal microbiota across multiple fields. However, this technique lacks standardization and requires careful experimental design in order to obtain optimal results. By comparing several studies in which rodents are the final recipients of FMT, we summarize the common practices employed. In this review, we document the limitations of this method and highlight different parameters to be considered while designing FMT Studies. Standardizing this method is challenging, as it differs according to the research topic, but avoiding common pitfalls is feasible. Several methodological questions remain unanswered to this day and we offer a discussion on issues to be explored in future studies. |
