Bromodomain-containing subunits BRD1, BRD2, and BRD13 are required for proper functioning of SWI/SNF complexes in Arabidopsis .

Autor:	Jarończyk K; Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-106 Warsaw, Poland., Sosnowska K; Institute of Biochemistry and Biophysics PAS, 02-106 Warsaw, Poland., Zaborowski A; Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-106 Warsaw, Poland., Pupel P; Department of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland., Bucholc M; Institute of Biochemistry and Biophysics PAS, 02-106 Warsaw, Poland., Małecka E; Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-106 Warsaw, Poland., Siwirykow N; Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-106 Warsaw, Poland., Stachula P; Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-106 Warsaw, Poland., Iwanicka-Nowicka R; Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-106 Warsaw, Poland.; Institute of Biochemistry and Biophysics PAS, 02-106 Warsaw, Poland., Koblowska M; Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-106 Warsaw, Poland.; Institute of Biochemistry and Biophysics PAS, 02-106 Warsaw, Poland., Jerzmanowski A; Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-106 Warsaw, Poland.; Institute of Biochemistry and Biophysics PAS, 02-106 Warsaw, Poland., Archacki R; Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-106 Warsaw, Poland.; Institute of Biochemistry and Biophysics PAS, 02-106 Warsaw, Poland.
Jazyk:	angličtina
Zdroj:	Plant communications [Plant Commun] 2021 Mar 05; Vol. 2 (4), pp. 100174. Date of Electronic Publication: 2021 Mar 05 (Print Publication: 2021).
DOI:	10.1016/j.xplc.2021.100174
Abstrakt:	SWI/SNF chromatin remodelers are evolutionarily conserved multiprotein complexes that use the energy of ATP hydrolysis to change chromatin structure. A characteristic feature of SWI/SNF remodelers is the occurrence in both the catalytic ATPase subunit and some auxiliary subunits, of bromodomains, the protein motifs capable of binding acetylated histones. Here, we report that the Arabidopsis bromodomain-containing proteins BRD1, BRD2, and BRD13 are likely true SWI/SNF subunits that interact with the core SWI/SNF components SWI3C and SWP73B. Loss of function of each single BRD protein caused early flowering but had a negligible effect on other developmental pathways. By contrast, a brd triple mutation ( brdx3 ) led to more pronounced developmental abnormalities, indicating functional redundancy among the BRD proteins. The brdx3 phenotypes, including hypersensitivity to abscisic acid and the gibberellin biosynthesis inhibitor paclobutrazol, resembled those of swi/snf mutants. Furthermore, the BRM protein level and occupancy at the direct target loci SCL3 , ABI5 , and SVP were reduced in the brdx3 mutant background. Finally, a brdx3 brm-3 quadruple mutant, in which SWI/SNF complexes were devoid of all constituent bromodomains, phenocopied a loss-of-function mutation in BRM. Taken together, our results demonstrate the relevance of BRDs as SWI/SNF subunits and suggest their cooperation with the bromodomain of BRM ATPase. (© 2021 The Authors.)
Databáze:	MEDLINE
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