Neuroprotective Effect of Taurine against Cell Death, Glial Changes, and Neuronal Loss in the Cerebellum of Rats Exposed to Chronic-Recurrent Neuroinflammation Induced by LPS.

Autor: Silva SP; Pathology Research Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite 245, CEP 90050-170 Porto Alegre, RS, Brazil., Zago AM; Pathology Research Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite 245, CEP 90050-170 Porto Alegre, RS, Brazil., Carvalho FB; Pathology Research Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite 245, CEP 90050-170 Porto Alegre, RS, Brazil., Germann L; Pathology Research Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite 245, CEP 90050-170 Porto Alegre, RS, Brazil., Colombo GM; Pathology Research Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite 245, CEP 90050-170 Porto Alegre, RS, Brazil., Rahmeier FL; Pathology Research Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite 245, CEP 90050-170 Porto Alegre, RS, Brazil., Gutierres JM; Pathology Research Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite 245, CEP 90050-170 Porto Alegre, RS, Brazil., Reschke CR; School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland.; FutureNeuro, The Science Foundation Ireland Research Centre for Chronic and Rare Neurological Diseases, RCSI University of Medicine and Health Sciences, Dublin, D02 YN77, Ireland., Bagatini MD; Graduate Program in Medical Sciences, Federal University of Fronteira Sul, Chapecó, SC, Brazil., Assmann CE; Postgraduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, Santa Maria, RS, Brazil., Fernandes MDC; Pathology Research Laboratory, Federal University of Health Sciences of Porto Alegre, Sarmento Leite 245, CEP 90050-170 Porto Alegre, RS, Brazil.
Jazyk: angličtina
Zdroj: Journal of immunology research [J Immunol Res] 2021 Jul 05; Vol. 2021, pp. 7497185. Date of Electronic Publication: 2021 Jul 05 (Print Publication: 2021).
DOI: 10.1155/2021/7497185
Abstrakt: The present study investigated the neuroprotective effect of taurine against the deleterious effects of chronic-recurrent neuroinflammation induced by LPS in the cerebellum of rats. Adult male Wistar rats were treated with taurine for 28 days. Taurine was administered at a dose of 30 or 100 mg/kg, by gavage. On days 7, 14, 21, and 28, the animals received LPS (250  μ g/kg) intraperitoneally. The vehicle used was saline. The animals were divided into six groups: vehicle, taurine 30 mg/kg, taurine 100 mg/kg, LPS, LPS plus taurine 30 mg/kg, and LPS plus taurine 100 mg/kg. On day 29, the animals were euthanized, and the cerebellum was removed and prepared for immunofluorescence analysis using antibodies of GFAP, NeuN, CD11b, and cleaved caspase-3. LPS group showed a reduction in the immunoreactivity of GFAP in the arbor vitae and medullary center and of NeuN in the granular layer of the cerebellar cortex. LPS increased the immunoreactivity of CD11b in the arbor vitae and in the medullary center. Taurine protected against these effects induced by LPS in immunoreactivity of GFAP, NeuN, and CD11b, with the 100 mg/kg dose being the most effective. LPS induced an increase in the number of positive cleaved caspase-3 cells in the Purkinje cell layers, granular layer, arbor vitae , and medullary center. Taurine showed its antiapoptotic activity by reducing the cleaved caspase-3 cells in relation to the LPS group. Here, a potential neuroprotective role of taurine can be seen since this amino acid was effective in protecting the cerebellum of rats against cell death and changes in glial and neuronal cells in the face of chronic-recurrent neuroinflammation.
Competing Interests: The authors declare that they have no competing interests.
(Copyright © 2021 Samara P. Silva et al.)
Databáze: MEDLINE
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