Cross-reactive antibodies against human coronaviruses and the animal coronavirome suggest diagnostics for future zoonotic spillovers.

Autor:	Klompus S; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel.; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel., Leviatan S; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel.; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel., Vogl T; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel thomas.vogl@weizmann.ac.il ziv.shulman@weizmann.ac.il eran.segal@weizmann.ac.il.; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel.; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel., Mazor RD; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel.; Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel., Kalka IN; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel.; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel.; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel., Stoler-Barak L; Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel.; Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel., Nathan N; Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel., Peres A; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel.; Faculty of Engineering, Bar Ilan University, Ramat Gan 52900, Israel., Moss L; Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel., Godneva A; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel.; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel., Kagan Ben Tikva S; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel.; Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel., Shinar E; Magen David Adom Blood Services, Israel., Dvashi HC; Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel., Gabizon R; Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel., London N; Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel., Diskin R; Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel., Yaari G; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel.; Faculty of Engineering, Bar Ilan University, Ramat Gan 52900, Israel., Weinberger A; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel.; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel.; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel., Shulman Z; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel thomas.vogl@weizmann.ac.il ziv.shulman@weizmann.ac.il eran.segal@weizmann.ac.il.; Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel., Segal E; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel thomas.vogl@weizmann.ac.il ziv.shulman@weizmann.ac.il eran.segal@weizmann.ac.il.; Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel.; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel.
Jazyk:	angličtina
Zdroj:	Science immunology [Sci Immunol] 2021 Jul 29; Vol. 6 (61).
DOI:	10.1126/sciimmunol.abe9950
Abstrakt:	The spillover of animal coronaviruses (aCoVs) to humans has caused SARS, MERS, and COVID-19. While antibody responses displaying cross-reactivity between SARS-CoV-2 and seasonal/common cold human coronaviruses (hCoVs) have been reported, potential cross-reactivity with aCoVs and the diagnostic implications are incompletely understood. Here, we probed for antibody binding against all seven hCoVs and 49 aCoVs represented as 12,924 peptides within a phage-displayed antigen library. Antibody repertoires of 269 recovered COVID-19 patients showed distinct changes compared to 260 unexposed pre-pandemic controls, not limited to binding of SARS-CoV-2 antigens but including binding to antigens from hCoVs and aCoVs with shared motifs to SARS-CoV-2. We isolated broadly reactive monoclonal antibodies from recovered COVID-19 patients that bind a shared motif of SARS-CoV-2, hCoV-OC43, hCoV-HKU1, and several aCoVs, demonstrating that interspecies cross-reactivity can be mediated by a single immunoglobulin. Employing antibody binding data against the entire CoV antigen library allowed accurate discrimination of recovered COVID-19 patients from unexposed individuals by machine learning. Leaving out SARS-CoV-2 antigens and relying solely on antibody binding to other hCoVs and aCoVs achieved equally accurate detection of SARS-CoV-2 infection. The ability to detect SARS-CoV-2 infection without knowledge of its unique antigens solely from cross-reactive antibody responses against other hCoVs and aCoVs suggests a potential diagnostic strategy for the early stage of future pandemics. Creating regularly updated antigen libraries representing the animal coronavirome can provide the basis for a serological assay already poised to identify infected individuals following a future zoonotic transmission event. (Copyright © 2021, American Association for the Advancement of Science.)
Databáze:	MEDLINE
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