Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts.
| Autor: | Legault EM; Faculté de Pharmacie, Université de Montréal, Montreal, QC, Canada., Drouin-Ouellet J; Faculté de Pharmacie, Université de Montréal, Montreal, QC, Canada. janelle.drouin-ouellet@umontreal.ca. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2352, pp. 97-115. |
| DOI: | 10.1007/978-1-0716-1601-7_7 |
| Abstrakt: | Since the first demonstration of direct dopaminergic neuronal reprogramming, over a dozen methods have been developed to generate induced dopaminergic neurons from various sources of cells. Here, we first present an overview of the different methods to generate induced neurons of a generic type and of different subtypes, with a particular focus on induced dopaminergic neurons generated from human fibroblasts. We then describe a protocol to generate induced dopaminergic neurons from commercially available human fetal lung fibroblasts. These cells could serve for various biomedical application, including regenerative medicine for conditions such as Parkinson's disease. (© 2021. Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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