An Exploratory Study Demonstrating That Salivary Cytokine Profiles Are Altered in Children With Small Area Thermal Injury.

Autor:	Carlton M; School of Biomedical Science, Faculty of Health, Queensland University of Technology, Brisbane, Queensland, Australia.; Centre for Children's Burn and Trauma Research, Centre for Children's Health Research, Queensland University of Technology, South Brisbane, Queensland, Australia., Voisey J; Centre for Genomics and Personalized Health, School of Biomedical Science, Faculty of Health, Queensland University of Technology, Australia., Jones L; Research Methods Group and Centre for Healthcare Transformation, School of Public Health and Social Work, Queensland University of Technology, Kelvin Grove, Queensland, Australia., Parker TJ; School of Biomedical Science, Faculty of Health, Queensland University of Technology, Brisbane, Queensland, Australia., Punyadeera C; Saliva and Liquid Biopsy Translational Research Team, School of Biomedical Sciences, Queensland University of Technology, Kelvin Grove, Queensland, Australia.; Translational Research Institute, Woolloongabba, Queensland, Australia., Cuttle L; School of Biomedical Science, Faculty of Health, Queensland University of Technology, Brisbane, Queensland, Australia.; Centre for Children's Burn and Trauma Research, Centre for Children's Health Research, Queensland University of Technology, South Brisbane, Queensland, Australia.
Jazyk:	angličtina
Zdroj:	Journal of burn care & research : official publication of the American Burn Association [J Burn Care Res] 2022 May 17; Vol. 43 (3), pp. 613-624.
DOI:	10.1093/jbcr/irab147
Abstrakt:	Serum can be used to investigate changes in cytokine concentration following burn injury in children; however, for children receiving treatment in an outpatient setting, blood is not routinely collected and therefore cannot be used for monitoring. The aim of this study was to investigate the use of saliva as a noninvasive tool for predicting burn outcomes by measuring the concentration of salivary cytokines in children with small area burns. A multiplex cytokine assay was used to measure 17 cytokines in the saliva of pediatric patients with burns (n = 20) and healthy controls (n = 20). After the removal of cytokines that had >30% of samples below the assay lower detection limit, six cytokines including IL-1β, IL-4, IL-7, IL-8, MCP-1, and TNFα were analyzed for association with burns. IL-1β and IL-4 were found to be significantly elevated in the pediatric burn patients compared to healthy controls. Interestingly, IL-1β was also significantly elevated in scald burns, compared to contact burns. In addition, biologically meaningful differences in cytokine concentration were identified in patients with different burn characteristics, which warrant further investigation. This exploratory study provides evidence that cytokines can be detected in the saliva of children and that salivary cytokine profiles differ between healthy controls and children with burns. Overall, this study demonstrates the value of saliva for the investigation of cytokines and its potential application in pediatric diagnostics, specifically in situations where blood collection is not appropriate. (© The Author(s) 2021. Published by Oxford University Press on behalf of the American Burn Association. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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