Real-Time Temperature Monitoring of Photoinduced Cargo Release inside Living Cells Using Hybrid Capsules Decorated with Gold Nanoparticles and Fluorescent Nanodiamonds.

Autor:	Gerasimova EN; Department of Physics and Engineering, ITMO University, Kronverksky Pr. 49, bldg. A, St. Petersburg 197101, Russian Federation., Yaroshenko VV; Department of Physics and Engineering, ITMO University, Kronverksky Pr. 49, bldg. A, St. Petersburg 197101, Russian Federation., Talianov PM; Department of Physics and Engineering, ITMO University, Kronverksky Pr. 49, bldg. A, St. Petersburg 197101, Russian Federation., Peltek OO; Department of Physics and Engineering, ITMO University, Kronverksky Pr. 49, bldg. A, St. Petersburg 197101, Russian Federation., Baranov MA; Faculty of Photonics and Optical Information, Center of Information Optical Technologies ITMO University, Kronverksky Pr. 49, bldg. A, St. Petersburg 197101, Russian Federation., Kapitanova PV; Department of Physics and Engineering, ITMO University, Kronverksky Pr. 49, bldg. A, St. Petersburg 197101, Russian Federation., Zuev DA; Department of Physics and Engineering, ITMO University, Kronverksky Pr. 49, bldg. A, St. Petersburg 197101, Russian Federation., Timin AS; Research School of Chemical and Biomedical Engineering, National Research Tomsk Polytechnic University, Tomsk 634050, Russian Federation.; R.M. Gorbacheva Research Institute for Pediatric Oncology, Hematology and Transplantation, Pavlov University, St. Petersburg 197022, Russian Federation., Zyuzin MV; Department of Physics and Engineering, ITMO University, Kronverksky Pr. 49, bldg. A, St. Petersburg 197101, Russian Federation.
Jazyk:	angličtina
Zdroj:	ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2021 Aug 11; Vol. 13 (31), pp. 36737-36746. Date of Electronic Publication: 2021 Jul 27.
DOI:	10.1021/acsami.1c05252
Abstrakt:	Real-time temperature monitoring within biological objects is a key fundamental issue for understanding the heating process and performing remote-controlled release of bioactive compounds upon laser irradiation. The lack of accurate thermal control significantly limits the translation of optical laser techniques into nanomedicine. Here, we design and develop hybrid (complex) carriers based on multilayered capsules combined with nanodiamonds (NV centers) as nanothermometers and gold nanoparticles (Au NPs) as nanoheaters to estimate an effective laser-induced temperature rise required for capsule rupture and further release of cargo molecules outside and inside cancerous (B16-F10) cells. We integrate both elements (NV centers and Au NPs) in the capsule structure using two strategies: (i) loading inside the capsule's cavity ( CORE ) and incorporating them inside the capsule's wall ( WALL ). Theoretically and experimentally, we show the highest and lowest heat release from capsule samples ( CORE or WALL ) under laser irradiation depending on the Au NP arrangement within the capsule. Applying NV centers, we measure the local temperature of capsule rupture inside and outside the cells, which is determined to be 128 ± 1.12 °C. Finally, the developed hybrid containers can be used to perform the photoinduced release of cargo molecules with simultaneous real-time temperature monitoring inside the cells.
Databáze:	MEDLINE
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