Methyl jasmonate-induced resistance to Delia platura (Diptera: Anthomyiidae) in Lupinus mutabilis.

Autor: Erazo-Garcia MP; Laboratorio de Biotecnología Agrícola y de Alimentos, Colegio de Ciencias e Ingenierías-Ing. en Agronomía, Universidad San Francisco de Quito, Quito, Ecuador., Sotelo-Proaño AR; Laboratorio de Entomología, Departamento de Protección Vegetal, Estación Experimental Santa Catalina, Instituto Nacional de Investigaciones Agropecuarias, Quito, Ecuador., Ramirez-Villacis DX; Laboratorio de Biotecnología Agrícola y de Alimentos, Colegio de Ciencias e Ingenierías-Ing. en Agronomía, Universidad San Francisco de Quito, Quito, Ecuador., Garcés-Carrera S; Laboratorio de Entomología, Departamento de Protección Vegetal, Estación Experimental Santa Catalina, Instituto Nacional de Investigaciones Agropecuarias, Quito, Ecuador., Leon-Reyes A; Laboratorio de Biotecnología Agrícola y de Alimentos, Colegio de Ciencias e Ingenierías-Ing. en Agronomía, Universidad San Francisco de Quito, Quito, Ecuador.
Jazyk: angličtina
Zdroj: Pest management science [Pest Manag Sci] 2021 Dec; Vol. 77 (12), pp. 5382-5395. Date of Electronic Publication: 2021 Aug 06.
DOI: 10.1002/ps.6578
Abstrakt: Background: Andean lupin (Lupinus mutabilis Sweet) is an important leguminous crop from South America with a high protein content. In Ecuador, lupin yields are severely affected by the infestation of Delia platura larvae on germinating seeds. The application of elicitor molecules with activity against herbivorous insects to control D. platura infestation constitutes an unexplored and promising alternative for chemical insecticides. In this study, methyl jasmonate (MeJA), hexanoic acid, menadione sodium bisulfite, and DL-β-aminobutyric acid were evaluated for their ability to induce resistance against D. platura in three commercial lupin cultivars.
Results: Only seeds pretreated with MeJA significantly impaired insect performance during choice and no-choice assays. Additionally, fitness indicators such as seed germination and growth were not affected by MeJA treatment. To investigate the molecular mechanisms behind the MeJA-mediated resistance, RT-qPCR assays were performed. First, RT-qPCR reference genes were validated, showing that LmUBC was the most stable reference gene. Next, expression analysis over time revealed that MeJA application up-regulated the activity of the jasmonic acid biosynthetic genes LmLOX2 and LmAOS, together with other jasmonate-related defense genes, such as LmTPS1, LmTPS4, LmPI2, LmMBL, LmL/ODC, LmCSD1, and LmPOD.
Conclusion: This study indicates that MeJA can be used as an environmentally friendly elicitor molecule to protect Andean lupin from D. platura attack without fitness cost. MeJA application induces plant defense responses to insects in Andean lupin that may be modulated by the onset of terpenoid biosynthesis, proteinase inhibitors, lectins, polyamines, and antioxidative enzymes. © 2021 Society of Chemical Industry.
(© 2021 Society of Chemical Industry.)
Databáze: MEDLINE
Externí odkaz: