Improvement of Fucosylated Oligosaccharides Synthesis by α-L-Fucosidase from Thermotoga maritima in Water-Organic Cosolvent Reaction System.

Autor: Robles-Arias MA; Departamento de Biotecnología, Universidad Autónoma Metropolitana, Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, 09340, Mexico City, Mexico., García-Garibay M; Departamento de Biotecnología, Universidad Autónoma Metropolitana, Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, 09340, Mexico City, Mexico.; Departamento de Ciencias de La Alimentación, Universidad Autónoma Metropolitan, Lerma, Av. Hidalgo Poniente 46, Col. La Estación, 52006, Lerma de Villada, State of Mexico, Mexico., Alatorre-Santamaría S; Departamento de Biotecnología, Universidad Autónoma Metropolitana, Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, 09340, Mexico City, Mexico., Tello-Solís SR; Departamento de Química, Universidad Autónoma Metropolitana, Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, 09340, Mexico City, Mexico., Guzmán-Rodriguez F; Departamento de Biotecnología, Universidad Autónoma Metropolitana, Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, 09340, Mexico City, Mexico., Gómez-Ruiz L; Departamento de Biotecnología, Universidad Autónoma Metropolitana, Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, 09340, Mexico City, Mexico., Rodríguez-Serrano G; Departamento de Biotecnología, Universidad Autónoma Metropolitana, Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, 09340, Mexico City, Mexico., Cruz-Guerrero AE; Departamento de Biotecnología, Universidad Autónoma Metropolitana, Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, 09340, Mexico City, Mexico. aec@xanum.uam.mx.
Jazyk: angličtina
Zdroj: Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2021 Nov; Vol. 193 (11), pp. 3553-3569. Date of Electronic Publication: 2021 Jul 26.
DOI: 10.1007/s12010-021-03628-3
Abstrakt: The effects of water activity (a w ), pH, and temperature on transglycosylation activity of α-L-fucosidase from Thermotoga maritima in the synthesis of fucosylated oligosaccharides were evaluated using different water-organic cosolvent reaction systems. The optimum conditions of transglycosylation reaction were the pH range between 7 and 10 and temperature 90-95 °C. The addition of organic cosolvent decreased α-L-fucosidase transglycosylation activity in the following order: acetone > dimethyl sulfoxide (DMSO) > acetonitrile (0.51 > 0.42 > 0.18 mM/h). However, the presence of DMSO and acetone enhanced enzyme-catalyzed transglycosylation over hydrolysis as demonstrated by the obtained transglycosylation/hydrolysis rate (r T/H ) values of 1.21 and 1.43, respectively. The lowest r T/H was calculated for acetonitrile (0.59), though all cosolvents tested improved the transglycosylation rate in comparison to a control assay (0.39). Overall, the study allowed the production of fucosylated oligosaccharides in water-organic cosolvent reaction media using α-L-fucosidase from T. maritima as biocatalyst.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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