Comparison of heat-inactivated and infectious SARS-CoV-2 across indoor surface materials shows comparable RT-qPCR viral signal intensity and persistence.

Autor: Salido RA; These authors contributed equally.; Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA., Cantú VJ; These authors contributed equally.; Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA., Clark AE; Division of Infectious Diseases and Global Public Health, Department of Medicine; University of California San Diego School of Medicine, 9500 Gilman Drive, La Jolla, California 92093, USA., Leibel SL; Department of Pediatrics, University of California San Diego, La Jolla, CA.; Sanford Consortium of Regenerative Medicine, University of California San Diego, La Jolla, CA., Foroughishafiei A; Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA., Saha A; Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA., Hakim A; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Nouri A; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Lastrella AL; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Castro-Martínez A; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Plascencia A; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Kapadia B; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Xia B; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Ruiz C; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Marotz CA; Department of Pediatrics, University of California San Diego, La Jolla, CA.; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Maunder D; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Lawrence ES; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Smoot EW; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Eisner E; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Crescini ES; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Kohn L; Herbert Wertheim School of Public Health, University of California, San Diego 9500 Gilman Drive, La Jolla, CA 92093., Vargas LF; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Chacón M; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Betty M; Department of Pediatrics, University of California San Diego, La Jolla, CA.; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA.; Rady Children's Hospital, San Diego, CA., Machnicki M; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Wu MY; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Baer NA; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Belda-Ferre P; Department of Pediatrics, University of California San Diego, La Jolla, CA.; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Hoff P; Sanford Consortium of Regenerative Medicine, University of California San Diego, La Jolla, CA.; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA.; Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Diego, USA., Seaver P; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Ostrander RT; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Tsai R; Department of Pediatrics, University of California San Diego, La Jolla, CA.; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Sathe S; Sanford Consortium of Regenerative Medicine, University of California San Diego, La Jolla, CA.; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA.; Dept of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA., Aigner S; Sanford Consortium of Regenerative Medicine, University of California San Diego, La Jolla, CA.; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA.; Dept of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA., Morgan SC; Sanford Consortium of Regenerative Medicine, University of California San Diego, La Jolla, CA.; Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Diego, USA., Ngo TT; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Barber T; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA., Cheung W; Sanford Consortium of Regenerative Medicine, University of California San Diego, La Jolla, CA.; Expedited COVID Identification Environment (EXCITE) Laboratory, Department of Pediatrics, University of California San Diego, La Jolla, CA.; San Diego State University, San Diego, CA., Carlin AF; Division of Infectious Diseases and Global Public Health, Department of Medicine; University of California San Diego School of Medicine, 9500 Gilman Drive, La Jolla, California 92093, USA., Yeo GW; Sanford Consortium of Regenerative Medicine, University of California San Diego, La Jolla, CA.; Dept of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA., Laurent LC; Sanford Consortium of Regenerative Medicine, University of California San Diego, La Jolla, CA.; Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Diego, USA., Fielding-Miller R; Herbert Wertheim School of Public Health, University of California, San Diego 9500 Gilman Drive, La Jolla, CA 92093.; Co-corresponding authors., Knight R; Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA.; Department of Pediatrics, University of California San Diego, La Jolla, CA.; Department of Computer Science and Engineering, University of California San Diego, La Jolla, CA, USA.; Center for Microbiome Innovation, Jacobs School of Engineering, University of California San Diego, La Jolla, CA, USA.; Co-corresponding authors.
Jazyk: angličtina
Zdroj: BioRxiv : the preprint server for biology [bioRxiv] 2021 Jul 19. Date of Electronic Publication: 2021 Jul 19.
DOI: 10.1101/2021.07.16.452756
Abstrakt: Environmental monitoring in public spaces can be used to identify surfaces contaminated by persons with COVID-19 and inform appropriate infection mitigation responses. Research groups have reported detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) on surfaces days or weeks after the virus has been deposited, making it difficult to estimate when an infected individual may have shed virus onto a SARS-CoV-2 positive surface, which in turn complicates the process of establishing effective quarantine measures. In this study, we determined that reverse transcription-quantitative polymerase chain reaction (RT-qPCR) detection of viral RNA from heat-inactivated particles experiences minimal decay over seven days of monitoring on eight out of nine surfaces tested. The properties of the studied surfaces result in RT-qPCR signatures that can be segregated into two material categories, rough and smooth, where smooth surfaces have a lower limit of detection. RT-qPCR signal intensity (average quantification cycle ( Cq )) can be correlated to surface viral load using only one linear regression model per material category. The same experiment was performed with infectious viral particles on one surface from each category, with essentially identical results. The stability of RT-qPCR viral signal demonstrates the need to clean monitored surfaces after sampling to establish temporal resolution. Additionally, these findings can be used to minimize the number of materials and time points tested and allow for the use of heat-inactivated viral particles when optimizing environmental monitoring methods.
Databáze: MEDLINE