Gene expression profiles of the small intestine of village chickens from an Ascaridia galli infested environment.

Autor: Malatji DP; Department of Agriculture and Animal Health, School of Agriculture and Consumer Science, University of South Africa, Johannesburg, South Africa., van Marle-Koster E; Department of Wildlife and Animal Science, Faculty of Natural and Agricultural Science, University of Pretoria, Pretoria, South Africa., Muchadeyi FC; Biotechnology Platform, Agricultural Research Council, Onderstepoort, South Africa. Electronic address: MuchadeyiF@arc.agric.za.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2019; Vol. 276S, pp. 100012. Date of Electronic Publication: 2019 May 21.
DOI: 10.1016/j.vpoa.2019.100012
Abstrakt: Nematodes of the genus Ascaridia are known to infect many species of birds and result in fatal diseases. A. galli damages the intestinal mucosa of chickens leading to blood loss, secondary infection and occasionally the obstruction of small intestines due to high worm burden. This study investigated the gene expression profiles in chickens from two different provinces of South Africa naturally exposed to A. galli infestations and tested either positive or negative for the parasite. The study further investigated gene expression profiles of the A. galli infected duodenum, jejunum and ileum tissues of the small intestines. The A. galli positive intestines displayed hypertrophy of the intestinal villi with accumulation of inflammatory cells and necrosis of the crypts of Lieberkühn glands, lesions that were absent in the uninfected intestines. Total RNA isolated from small intestines of infected and non-infected intestines was sequenced using Illumina HiSeq technology to generate up to 23,856,130 reads. Between any two-way comparisons of the intestines, 277 and 190 transcripts were significantly expressed in Limpopo and KwaZulu-Natal (KZN) chickens, respectively. Gene ontology analysis of the differentially expressed genes (DEGs) revealed an enrichment of genes reported to function in the immune response, defense response, inflammatory response and cell signalling genes. T cell receptor signalling pathways and arachidonic acid metabolism pathways were among the most significantly impacted pathways. Overall, the study provided insights into adaptative mechanisms for chickens extensively raised in parasite infected environments.
(Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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