Analysis and effective separation of toxic pollutants from water resources using MBBR: Pathway prediction using alkaliphilic P. mendocina.

Autor: Sridharan R; Department of Biotechnology, Stella Maris College (Autonomous) Affiliated to University of Madras, Chennai, Tamil Nadu 600 086, India., Krishnaswamy V; Department of Biotechnology, Stella Maris College (Autonomous) Affiliated to University of Madras, Chennai, Tamil Nadu 600 086, India. Electronic address: veenagayathri@stellamariscollege.edu.in., Kumar PS; Department of Chemical Engineering, Sri Sivasubramaniya Nadar College of Engineering, Chennai 603110, India; Centre of Excellence in Water Research (CEWAR), Sri Sivasubramaniya Nadar College of Engineering, Chennai 603110, India. Electronic address: senthilkumarp@ssn.edu.in., Vidhya TA; Department of Biotechnology, Stella Maris College (Autonomous) Affiliated to University of Madras, Chennai, Tamil Nadu 600 086, India., Sivamurugan V; Department of Chemistry, Pachaiyappa's College, Chennai, Tamil Nadu 600 030, India., Kumar DT; Department of Bioinformatics, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Chennai 602 105, India., Doss CGP; School of BioSciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu 632 014, India., Vo DN; Institute of Environmental Sciences, Nguyen Tat Thanh University, Ho Chi Minh City, Viet Nam.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2021 Nov 25; Vol. 797, pp. 149135. Date of Electronic Publication: 2021 Jul 20.
DOI: 10.1016/j.scitotenv.2021.149135
Abstrakt: Azo dyes are highly toxic, which acts as a notable mutagen and carcinogen. This has a significant effect on human health, plants, animals, aquatic and terrestrial environments. Thus, the degradation of the azo dyes is exclusively studied using the conventional methods of which biodegradation is an eco-friendly approach. Hence, the present study is focused on the elucidation of reactive mixed azo dye degradation pathway using MBBR and laccase enzyme produced by an alkaliphilic bacterium P. mendocina. Synthetic wastewater treatment performed using MBBR was very effective which reduced the COD and BOD to 90 mg/L and 460 mg/L. The potential degrader P. mendocina was isolated and laccase enzyme was screened. Finally, the degradation pathway was elucidated. The in silico toxicity analysis predicted Reactive Red and Reactive Brown as developmental toxicants during Reactive Black as Developmental non-toxicant. Docking studies were performed to understand interaction of laccase with compounds evolved from dyes.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021. Published by Elsevier B.V.)
Databáze: MEDLINE
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