Direct-MS analysis of antibody-antigen complexes.

Autor: Vimer S; Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel., Ben-Nissan G; Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel., Marty M; Department of Chemistry & Biochemistry, University of Arizona, Tucson, Arizona, USA., Fleishman SJ; Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel., Sharon M; Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot, Israel.
Jazyk: angličtina
Zdroj: Proteomics [Proteomics] 2021 Nov; Vol. 21 (21-22), pp. e2000300. Date of Electronic Publication: 2021 Aug 09.
DOI: 10.1002/pmic.202000300
Abstrakt: In recent decades, antibodies (Abs) have attracted the attention of academia and the biopharmaceutical industry due to their therapeutic properties and versatility in binding a vast spectrum of antigens. Different engineering strategies have been developed for optimizing Ab specificity, efficacy, affinity, stability and production, enabling systematic screening and analysis procedures for selecting lead candidates. This quality assessment is critical but usually demands time-consuming and labor-intensive purification procedures. Here, we harnessed the direct-mass spectrometry (direct-MS) approach, in which the analysis is carried out directly from the crude growth media, for the rapid, structural characterization of designed Abs. We demonstrate that properties such as stability, specificity and interactions with antigens can be defined, without the need for prior purification.
(© 2021 Wiley-VCH GmbH.)
Databáze: MEDLINE
Externí odkaz: