Testicular morphometry of rats with Walker 256 tumor supplemented with L-glutamine.

Autor:	Rocha NR; Departamento de Morfologia, Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil., Braz JKFDS; Departamento de Morfologia, Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil., de Souza SRG; Departamento de Ciências Morfológicas, Universidade Estadual de Maringá, Maringá, PR, Brasil., Fracaro L; Departamento de Ciências Morfológicas, Universidade Estadual de Maringá, Maringá, PR, Brasil., de Melo FCSA; Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa MG, Brasil., Zanoni JN; Departamento de Ciências Morfológicas, Universidade Estadual de Maringá, Maringá, PR, Brasil., Clebis NK; Departamento de Morfologia, Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil., Morais DB; Departamento de Morfologia, Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil., de Moura CEB; Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brasil.
Jazyk:	angličtina
Zdroj:	Animal reproduction [Anim Reprod] 2021 Jul 19; Vol. 18 (2), pp. e20200051. Date of Electronic Publication: 2021 Jul 19 (Print Publication: 2021).
DOI:	10.1590/1984-3143-AR2020-0051
Abstrakt:	Glutamine is often used to treat metabolic changes associated with anorexia-cachexia syndrome in patients with malignant neoplasms. Walker 256 tumor is an excellent model for studying these changes associated with cancer in different organs, including injuries in testicular functions. However, the effects of supplementing glutamine on testicular morphometry in this model have not yet been investigated. Thus, the objective of this study was to evaluate the effect of L-glutamine supplementation on testicular morphometry in rats transplanted with Walker 256 tumor cells. Forty puberty Wistar rats were divided into four groups: control without L-glutamine (C); control supplemented with L-glutamine (CG); inoculated with Walker 256 tumor cells (WT) and inoculated with Walker 256 tumor cells and supplemented with L-glutamine (WTG). The testicles were removed, weighed, fixed in Bouin, and included in paraffin for histomorphometric analysis. Walker 256 tumor caused quantitative changes in the tubular and intertubular compartments and tunica albuginea, with reductions in the percentages of lumen and tunica albuginea, number of Sertoli cells per gram of testis; number of Leydig cells; percentage of blood vessels and connective tissue in intertubule. However, glutamine supplementation prevented part of these changes caused by the tumor, presenting mainly a protective effect on the tunica albuginea and percentage of blood and lymph vessels in the intertubule. These results indicate the potential of L-glutamine was able to recover for testicular dysfunction associated with cancer. Competing Interests: Conflicts of interest: The authors have no conflict of interest to declare. (Copyright © The Author(s).)
Databáze:	MEDLINE
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