Rat model of smoke inhalation-induced acute lung injury.

Autor: Leiphrakpam PD; Surgery, University of Nebraska Medical Center, Omaha, Nebraska, USA., Weber HR; Surgery, University of Nebraska Medical Center, Omaha, Nebraska, USA., Ogun T; Family Medicine, University of Nebraska Medical Center, Omaha, Nebraska, USA., Buesing KL; Surgery, University of Nebraska Medical Center, Omaha, Nebraska, USA keely.buesing@unmc.edu.
Jazyk: angličtina
Zdroj: BMJ open respiratory research [BMJ Open Respir Res] 2021 Jul; Vol. 8 (1).
DOI: 10.1136/bmjresp-2021-000879
Abstrakt: Background: Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a lethal disease with limited therapeutic options and an unacceptably high mortality rate. Understanding the complex pathophysiological processes involved in the development of ALI/ARDS is critical for developing novel therapeutic strategies. Smoke inhalation (SI) injury is the leading cause of morbidity and mortality in patients with burn-associated ALI/ARDS; however, to our knowledge few reliable, reproducible models are available for pure SI animal model to investigate therapeutic options for ALI/ARDS without the confounding variables introduced by cutaneous burn or other pathology.
Objective: To develop a small animal model of pure SI-induced ALI and to use this model for eventual testing of novel therapeutics for ALI.
Methods: Rats were exposed to smoke using a custom-made smoke generator. Peripheral oxygen saturation (SpO 2 ), heart rate, arterial blood gas, and chest X-ray (CXR) were measured before and after SI. Wet/dry weight (W/D) ratio, lung injury score and immunohistochemical staining of cleaved caspase 3 were performed on harvested lung tissues of healthy and SI animals.
Results: The current study demonstrates the induction of ALI in rats after SI as reflected by a significant, sustained decrease in SpO 2 and the development of diffuse bilateral pulmonary infiltrates on CXR. Lung tissue of animals exposed to SI showed increased inflammation, oedema and apoptosis as reflected by the increase in W/D ratio, injury score and cleaved caspase 3 level of the harvested tissues compared with healthy animals.
Conclusion: We have successfully developed a small animal model of pure SI-induced ALI. This model is offered to the scientific community as a reliable model of isolated pulmonary SI-induced injury without the confounding variables of cutaneous injury or other systemic pathology to be used for study of novel therapeutics or other investigation.
Competing Interests: Competing interests: None declared.
(© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
Databáze: MEDLINE
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