A Miniaturized Microbe-Silicon-Chip Based on Bioluminescent Engineered Escherichia coli for the Evaluation of Water Quality and Safety.
| Autor: | Sciuto EL; Azienda Ospedaliero Universitaria Policlinico 'G. Rodolico-San Marco', Via S. Sofia 78, 95123 Catania, Italy., Corso D; Istituto per la Microelettronica e Microsistemi-Consiglio Nazionale delle Ricerche (CNR-IMM), Ottava Strada 5, 95121 Catania, Italy., Libertino S; Istituto per la Microelettronica e Microsistemi-Consiglio Nazionale delle Ricerche (CNR-IMM), Ottava Strada 5, 95121 Catania, Italy., van der Meer JR; Department of Fundamental Microbiology, Bâtiment Biophore, University of Lausanne, 1015 Lausanne, Switzerland., Faro G; Azienda Sanitaria Provinciale di Catania, Via S. Maria La Grande 5, 95124 Catania, Italy., Coniglio MA; Azienda Ospedaliero Universitaria Policlinico 'G. Rodolico-San Marco', Via S. Sofia 78, 95123 Catania, Italy.; Istituto per la Microelettronica e Microsistemi-Consiglio Nazionale delle Ricerche (CNR-IMM), Ottava Strada 5, 95121 Catania, Italy.; Regional Reference Laboratory of Clinical and Environmental Surveillance of Legionellosis, Catania, Department of Medical and Surgical Sciences and Advanced Technologies 'G.F. Ingrassia', University of Catania, Via Sofia 87, 95123 Catania, Italy. |
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| Jazyk: | angličtina |
| Zdroj: | International journal of environmental research and public health [Int J Environ Res Public Health] 2021 Jul 16; Vol. 18 (14). Date of Electronic Publication: 2021 Jul 16. |
| DOI: | 10.3390/ijerph18147580 |
| Abstrakt: | Conventional high throughput methods assaying the chemical state of water and the risk of heavy metal accumulation share common constraints of long and expensive analytical procedures and dedicated laboratories due to the typical bulky instrumentation. To overcome these limitations, a miniaturized optical system for the detection and quantification of inorganic mercury (Hg 2+ ) in water was developed. Combining the bioactivity of a light-emitting mercury-specific engineered Escherichia coli -used as sensing element-with the optical performance of small size and inexpensive Silicon Photomultiplier (SiPM)-used as detector-the system is able to detect mercury in low volumes of water down to the concentration of 1 µg L -1 , which is the tolerance value indicated by the World Health Organization (WHO), providing a highly sensitive and miniaturized tool for in situ water quality analysis. |
| Databáze: | MEDLINE |
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