| Autor: |
Olmos Calvo I; OrthoSera GmbH, Dr. Karl-Dorrek-Straße 23-29, 3500 Krems an der Donau, Austria., Kuten-Pella O; OrthoSera GmbH, Dr. Karl-Dorrek-Straße 23-29, 3500 Krems an der Donau, Austria., Kramer K; Center for Regenerative Medicine, Danube University of Krems, 3500 Krems an der Donau, Austria., Madár Á; OrthoSera GmbH, Dr. Karl-Dorrek-Straße 23-29, 3500 Krems an der Donau, Austria., Takács S; OrthoSera GmbH, Dr. Karl-Dorrek-Straße 23-29, 3500 Krems an der Donau, Austria., Kardos D; Research Center for Natural Sciences, 1117 Budapest, Hungary., Simon D; Department of Immunology and Biotechnology, Medical School, University of Pécs, 7624 Pécs, Hungary., Erdö-Bonyár S; Department of Immunology and Biotechnology, Medical School, University of Pécs, 7624 Pécs, Hungary., Berki T; Department of Immunology and Biotechnology, Medical School, University of Pécs, 7624 Pécs, Hungary., De Luna A; Center for Regenerative Medicine, Danube University of Krems, 3500 Krems an der Donau, Austria., Nehrer S; Center for Regenerative Medicine, Danube University of Krems, 3500 Krems an der Donau, Austria., Lacza Z; Department of Sport Physiology, University of Physical Education, 1123 Budapest, Hungary.; Institute of Translational Medicine, Semmelweis University, 1085 Budapest, Hungary. |
| Abstrakt: |
Hyperacute serum (HAS) is a blood derivative product that promotes the proliferation of various cell types and controls inflammation in vitro. The aim of this study is to investigate the regenerative potential of different formulations of HAS, including lyophilized and hyaluronic acid combined versions, to obtain a stable and standardized therapeutic in osteoarthritis (OA), which may be able to overcome the variability limitations of platelet-rich plasma (PRP). Primary human osteoarthritic chondrocytes were used for testing cellular viability and gene expression of OA-related genes. Moreover, a co-culture of human explants of cartilage, bone and synovium under inflammatory conditions was used for investigating the inflammatory control capacities of the different therapeutics. In this study, one formulation of lyophilized HAS achieved the high cell viability rates of liquid HAS and PRP. Gene expression analysis showed that HAS induced higher Col1a1 expression than PRP. Cytokine quantification from supernatant fluids revealed that HAS treatment of inflamed co-cultures significantly reduced levels of IL-5, IL-15, IL-2, TNFα, IL-7 and IL-12. To conclude, lyophilized HAS is a stable and standardized therapeutic with high potential in joint regeneration. |
