Evidence for the existence of a new genus Chlamydiifrater gen. nov. inside the family Chlamydiaceae with two new species isolated from flamingo (Phoenicopterus roseus): Chlamydiifrater phoenicopteri sp. nov. and Chlamydiifrater volucris sp. nov.

Autor: Vorimore F; University Paris-Est, Anses, Animal Health Laboratory, Bacterial Zoonoses Unit, Maisons-Alfort, France. Electronic address: fabien.vorimore@anses.fr., Hölzer M; Robert Koch Institute, MF1 Bioinformatics, Berlin, Germany., Liebler-Tenorio EM; Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Institute of Molecular Pathogenesis, Germany., Barf LM; Friedrich-Schiller-Universität Jena, RNA Bioinformatics and High-Throughput Analysis, Jena, Germany., Delannoy S; University Paris-Est, Anses, Food Research Laboratory, IdentyPath Platform, Maisons-Alfort, France., Vittecoq M; Tour du Valat, Centre de recherche pour la conservation des zones humides méditerranéennes, Le Sambuc, Arles, France., Wedlarski R; Bioparc - Zoo de Doué la fontaine, 103 rue de Cholet, 49700 Doué la Fontaine, France., Lécu A; Parc Zoologique de Paris, avenue de Daumesnil, 75012 Paris, France., Scharf S; Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Institute of Molecular Pathogenesis, Germany., Blanchard Y; Unit of Viral Genetics and Biosafety, ANSES, Laboratory of Ploufragan, Ploufragan, France., Fach P; University Paris-Est, Anses, Food Research Laboratory, IdentyPath Platform, Maisons-Alfort, France., Hsia RC; University of Maryland, Electron Microscopy Core Imaging Facility, Baltimore, MD 21201, USA., Bavoil PM; University of Maryland, Department of Microbial Pathogenesis, Baltimore, MD 21201, USA., Rosselló-Móra R; Marine Microbiology Group, Department of Animal and Microbial Biodiversity, Mediterranean Institute for Advanced Studies, 07190 Esporles, Spain., Laroucau K; University Paris-Est, Anses, Animal Health Laboratory, Bacterial Zoonoses Unit, Maisons-Alfort, France., Sachse K; Friedrich-Schiller-Universität Jena, RNA Bioinformatics and High-Throughput Analysis, Jena, Germany.
Jazyk: angličtina
Zdroj: Systematic and applied microbiology [Syst Appl Microbiol] 2021 Jul; Vol. 44 (4), pp. 126200. Date of Electronic Publication: 2021 Mar 29.
DOI: 10.1016/j.syapm.2021.126200
Abstrakt: The family Chlamydiaceae currently comprises a single genus Chlamydia, with 11 validly published species and seven more taxa. It includes the human pathogens Chlamydia (C.) trachomatis, C. pneumoniae and C. psittaci, a zoonotic agent causing avian chlamydiosis and human psittacosis, as well as other proven or potential pathogens in ruminants, birds, snakes, reptiles and turtles. During routine testing of 15 apparently healthy captive flamingos in a zoo in 2011, an atypical strain of Chlamydiaceae was detected by real-time PCR of cloacal swab samples. Sequence analysis of the 16S rRNA gene revealed high similarity to the uncultured Chlamydiales bacterium clone 122, which previously had been found in gulls. As more samples were collected during annual campaigns of the flamingo ringing program in southern France from 2012 to 2015, Chlamydiaceae-specific DNA was detected by PCR in 30.9% of wild birds. From these samples, three strains were successfully grown in cell culture. Ultrastructural analysis, comparison of 16S and 23S rRNA gene sequences, whole-genome analysis based on de novo hybrid-assembled sequences of the new strains as well as subsequent calculation of taxonomic parameters revealed that the relatedness of the flamingo isolates to established members of the family Chlamydiaceae was sufficiently distant to indicate that the three strains belong to two distinct species within a new genus. Based on these data, we propose the introduction of Chlamydiifrater gen. nov., as a new genus, and Chlamydiifrater phoenicopteri sp. nov. and Chlamydiifrater volucris sp. nov., as two new species of the genus.
(Copyright © 2021 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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