Toxicity and genotoxicity of domestic sewage sludge in the freshwater snail Biomphalaria glabrata (Say, 1818).

Autor: de Siqueira WN; Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil. williams.wns@gmail.com.; Serviço de Monitoração Ambiental, Centro Regional de Ciências Nucleares do Nordeste, Recife, Pernambuco, Brazil. williams.wns@gmail.com.; Laboratório de Radiobiologia, Universidade Federal de Pernambuco, Av. Prof. Moraes Rêgo, s/n, Recife, PE, 50780-901, Brazil. williams.wns@gmail.com., de França EJ; Serviço de Monitoração Ambiental, Centro Regional de Ciências Nucleares do Nordeste, Recife, Pernambuco, Brazil., Pereira DR; Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Lima MV; Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.; Serviço de Monitoração Ambiental, Centro Regional de Ciências Nucleares do Nordeste, Recife, Pernambuco, Brazil., Silva HAMF; Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Sá JLF; Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., de Araújo HDA; Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Melo AMMA; Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2021 Dec; Vol. 28 (48), pp. 69343-69353. Date of Electronic Publication: 2021 Jul 22.
DOI: 10.1007/s11356-021-15529-3
Abstrakt: Waste produced in homes is one of the main sources of pollutants in freshwater ecosystems. Therefore, it is imperative to implement methodologies that aid in environmental monitoring procedures. The use of organisms as biomonitors has grown increasingly prevalent as they are models that provide data that can be adequately evaluated. In this work, we investigated the genotoxic and cytotoxic effects caused by domestic sewage sludge through an analysis of biomarkers in the mollusk Biomphalaria glabrata. For the tests, increasing concentrations of 50, 100, 150, and 500 mg L -1 of domestic sewage sludge were standardized, in addition to control groups. Assays were performed after the mollusks were exposed to the domestic sewage sludge in acute (48 h) and chronic (15 d) manner. Toxicity tests were performed with embryonic and adult snails. The cytoplasmic and nuclear changes were analyzed in the hemocyte cells. Lastly, genotoxic damage was analyzed using the comet assay. Adult snails and embryos of B. glabrata showed no significant morphological changes. Domestic sludge caused deleterious effects on mollusks as confirmed after cell genotoxicity tests. Therefore, based on the results obtained from the analysis of B. glabrata hemocytes, we can affirm that domestic sewage sludge causes genotoxic and cytotoxic effects on mollusk cells. Therefore, it is possible to conclude that the mollusk Biomphalaria glabrata can be used as a good low-cost alternative to assist in the biomonitoring of freshwater environments. Graphical Abstract.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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