RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages.

Autor:	Valečka J; Aix Marseille Université, CNRS, INSERM, CIML, 13288 Marseille cedex 9, France., Camosseto V; Aix Marseille Université, CNRS, INSERM, CIML, 13288 Marseille cedex 9, France., McEwan DG; Tumour Cell Death Laboratory, Cancer Research UK Beatson Institute, Garscube Estate, Switchback Road, Glasgow G61 1BD, UK., Terawaki S; Department of Pathobiochemistry, Graduate School of Medicine, Osaka City University, Osaka, Japan., Liu Z; School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, People's Republic of China., Strock E; Aix Marseille Université, CNRS, INSERM, CIML, 13288 Marseille cedex 9, France., Almeida CR; Institute for Research in Biomedicine (iBiMED) and Ilidio Pinho Foundation, Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, Portugal., Su B; Shanghai Institute of Immunology, Department of Microbiology and Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People's Republic of China., Dikic I; Institute of Biochemistry II, Goethe University School of Medicine, Theodor-Stern-Kai 7, 60590 Frankfurt (Main), Germany., Liang Y; School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, People's Republic of China., Gatti E; Aix Marseille Université, CNRS, INSERM, CIML, 13288 Marseille cedex 9, France.; Institute for Research in Biomedicine (iBiMED) and Ilidio Pinho Foundation, Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, Portugal., Pierre P; Aix Marseille Université, CNRS, INSERM, CIML, 13288 Marseille cedex 9, France.; Institute for Research in Biomedicine (iBiMED) and Ilidio Pinho Foundation, Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, Portugal.; Shanghai Institute of Immunology, Department of Microbiology and Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People's Republic of China.
Jazyk:	angličtina
Zdroj:	Royal Society open science [R Soc Open Sci] 2021 Jul 14; Vol. 8 (7), pp. 202333. Date of Electronic Publication: 2021 Jul 14 (Print Publication: 2021).
DOI:	10.1098/rsos.202333
Abstrakt:	We report here that RUFY4, a newly characterized member of the 'RUN and FYVE domain-containing' family of proteins previously associated with autophagy enhancement, is highly expressed in alveolar macrophages (AM). We show that RUFY4 interacts with mitochondria upon stimulation by microbial-associated molecular patterns of AM and dendritic cells. RUFY4 interaction with mitochondria and other organelles is dependent on a previously uncharacterized OmpH domain located immediately upstream of its C-terminal FYVE domain. Further, we demonstrate that rufy4 messenger RNA can be translated from an alternative translation initiation codon, giving rise to a N-terminally truncated form of the molecule lacking most of its RUN domain and with enhanced potential for its interaction with mitochondria. Our observations point towards a role of RUFY4 in selective mitochondria clearance in activated phagocytes. (© 2021 The Authors.)
Databáze:	MEDLINE
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