Intramuscular Injection of miR-1 Reduces Insulin Resistance in Obese Mice.

Autor:	Rodrigues AC; Department of Pharmacology, Instituto de Ciencias Biomedicas, Universidade de São Paulo, São Paulo, Brazil., Spagnol AR; Department of Pharmacology, Instituto de Ciencias Biomedicas, Universidade de São Paulo, São Paulo, Brazil., Frias FT; Department of Pharmacology, Instituto de Ciencias Biomedicas, Universidade de São Paulo, São Paulo, Brazil., de Mendonça M; Department of Pharmacology, Instituto de Ciencias Biomedicas, Universidade de São Paulo, São Paulo, Brazil., Araújo HN; Obesity and Comorbidities Research Center (OCRC), Campinas, Brazil.; Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil., Guimarães D; Obesity and Comorbidities Research Center (OCRC), Campinas, Brazil.; Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil., Silva WJ; Department of Anatomy, Instituto de Ciencias Biomedicas, Universidade de São Paulo, São Paulo, Brazil., Bolin AP; Department of Pharmacology, Instituto de Ciencias Biomedicas, Universidade de São Paulo, São Paulo, Brazil., Murata GM; Department of Medical Clinics, Faculty of Medicine, University of São Paulo, São Paulo, Brazil., Silveira L; Obesity and Comorbidities Research Center (OCRC), Campinas, Brazil.; Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in physiology [Front Physiol] 2021 Jul 06; Vol. 12, pp. 676265. Date of Electronic Publication: 2021 Jul 06 (Print Publication: 2021).
DOI:	10.3389/fphys.2021.676265
Abstrakt:	The role of microRNAs in metabolic diseases has been recognized and modulation of them could be a promising strategy to treat obesity and obesity-related diseases. The major purpose of this study was to test the hypothesis that intramuscular miR-1 precursor replacement therapy could improve metabolic parameters of mice fed a high-fat diet. To this end, we first injected miR-1 precursor intramuscularly in high-fat diet-fed mice and evaluated glucose tolerance, insulin sensitivity, and adiposity. miR-1-treated mice did not lose weight but had improved insulin sensitivity measured by insulin tolerance test. Next, using an in vitro model of insulin resistance by treating C2C12 cells with palmitic acid (PA), we overexpressed miR-1 and measured p-Akt content and the transcription levels of a protein related to fatty acid oxidation. We found that miR-1 could not restore insulin sensitivity in C2C12 cells, as indicated by p-Akt levels and that miR-1 increased expression of Pgc1a and Cpt1b in PA-treated cells, suggesting a possible role of miR-1 in mitochondrial respiration. Finally, we analyzed mitochondrial oxygen consumption in primary skeletal muscle cells treated with PA and transfected with or without miR-1 mimic. PA-treated cells showed reduced basal respiration, oxygen consumption rate-linked ATP production, maximal and spare capacity, and miR-1 overexpression could prevent impairments in mitochondrial respiration. Our data suggest a role of miR-1 in systemic insulin sensitivity and a new function of miR-1 in regulating mitochondrial respiration in skeletal muscle. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Rodrigues, Spagnol, Frias, de Mendonça, Araújo, Guimarães, Silva, Bolin, Murata and Silveira.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu