Cerebrospinal Fluid of Patients With Alzheimer's Disease Contains Increased Percentages of Synaptophysin-Bearing Microvesicles.

Autor:	Utz J; Department of Psychiatry and Psychotherapy, Friedrich-Alexander-University of Erlangen-Nuremberg (FAU), Erlangen, Germany., Berner J; Department of Psychiatry and Psychotherapy, Friedrich-Alexander-University of Erlangen-Nuremberg (FAU), Erlangen, Germany., Muñoz LE; Department of Internal Medicine, Friedrich-Alexander-University of Erlangen-Nuremberg (FAU), Erlangen, Germany.; Department of Rheumatology and Immunology, Friedrich-Alexander-University of Erlangen-Nuremberg (FAU), Erlangen, Germany., Oberstein TJ; Department of Psychiatry and Psychotherapy, Friedrich-Alexander-University of Erlangen-Nuremberg (FAU), Erlangen, Germany., Kornhuber J; Department of Psychiatry and Psychotherapy, Friedrich-Alexander-University of Erlangen-Nuremberg (FAU), Erlangen, Germany., Herrmann M; Department of Internal Medicine, Friedrich-Alexander-University of Erlangen-Nuremberg (FAU), Erlangen, Germany.; Department of Rheumatology and Immunology, Friedrich-Alexander-University of Erlangen-Nuremberg (FAU), Erlangen, Germany., Maler JM; Department of Psychiatry and Psychotherapy, Friedrich-Alexander-University of Erlangen-Nuremberg (FAU), Erlangen, Germany., Spitzer P; Department of Psychiatry and Psychotherapy, Friedrich-Alexander-University of Erlangen-Nuremberg (FAU), Erlangen, Germany.
Jazyk:	angličtina
Zdroj:	Frontiers in aging neuroscience [Front Aging Neurosci] 2021 Jul 06; Vol. 13, pp. 682115. Date of Electronic Publication: 2021 Jul 06 (Print Publication: 2021).
DOI:	10.3389/fnagi.2021.682115
Abstrakt:	Introduction: In Alzheimer's disease, the severity of symptoms is linked to a loss of synaptic density and the spread of pathologically hyperphosphorylated tau. The established cerebrospinal fluid markers Aβ, tau and phospho-tau reflect the histopathological hallmarks of Alzheimer's disease but do not indicate disease progression. Such markers are of special interest, especially for trials of disease modifying drugs. Microvesicles are produced by stressed cells and reflect part of the metabolism of their cells of origin. Therefore, we investigated microvesicles of neuronal origin in cerebrospinal fluid. Materials and Methods: We used flow cytometry to analyze microvesicles carrying tau, phospho-tau-Thr181, phospho-tau-Ser202Thr205, synaptophysin, and SNAP-25 in the cerebrospinal fluid of 19 patients with Alzheimer's disease and 15 non-inflammatory neurological disease controls. Results: The percentages of synaptophysin-bearing microvesicles were significantly higher in the cerebrospinal fluid of patients with Alzheimer's disease than in the CSF of non-inflammatory neurological disease controls. Tau, phospho-tau-Thr181, phospho-tau-Ser202Thr205, and SNAP-25 did not differ between the groups. The percentages of synaptophysin-bearing vesicles distinguished patients with Alzheimer's disease from the controls (AUC = 0.81). Conclusion: The loss of synapses in Alzheimer's disease may be reflected by synaptophysin-bearing microvesicles in the cerebrospinal fluid. Future studies are needed to investigate the possibility of using these MVs as a marker to determine the activity of Alzheimer's disease. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Utz, Berner, Muñoz, Oberstein, Kornhuber, Herrmann, Maler and Spitzer.)
Databáze:	MEDLINE
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