| Autor: |
Aebig JA; American Type Culture Collection, Rockville, Md., Jordan RL, Lawson RH, Hsu HT |
| Jazyk: |
angličtina |
| Zdroj: |
Intervirology [Intervirology] 1987; Vol. 28 (1), pp. 57-64. |
| DOI: |
10.1159/000149997 |
| Abstrakt: |
A monoclonal antibody reacting with prunus necrotic ringspot ilarvirus was tested in immunochemical studies, neutralization of infectivity assays, and by immuno-electron microscopy. The antibody was able to detect the 27,000 Mr coat protein of prunus necrotic ringspot ilarvirus in western blots and also detected all polypeptide fragments generated after incubation of whole virus with proteolytic enzymes. In neutralization of infectivity studies, the antibody blocked virus infectivity, although it did not precipitate the antigen in agar gel Ouchterlony double diffusion tests. Immuno-electron microscopy confirmed that the antibody coats virions but does not cause clumping. The antibody may be a useful tool for investigating coat protein-dependent initiation of ilarvirus infection. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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