Identification of Bacillus anthracis, Brucella spp., and Coxiella burnetii DNA signatures from bushmeat.

Autor: Katani R; Applied Biological and Biosecurity Research Laboratory, Pennsylvania State University, University Park, PA, USA.; The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA., Schilling MA; The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA.; Department of Animal Science, Pennsylvania State University, University Park, PA, USA., Lyimo B; Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania., Eblate E; Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania.; Tanzania Wildlife Research Institute, Arusha, Tanzania., Martin A; Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania., Tonui T; Biosciences Eastern and Central Africa-International Livestock Research Institute (BecA-ILRI) Hub, Nairobi, Kenya., Cattadori IM; The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA.; Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania.; Department of Biology, Pennsylvania State University, University Park, PA, USA., Francesconi SC; Naval Medical Research Center, Fort Detrick, MD, USA., Estes AB; The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA.; Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania.; Department of Environmental Studies, Carleton College, Northfield, MN, USA., Rentsch D; Lincoln Park Zoo, Chicago, IL, USA., Srinivasan S; The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA., Lyimo S; Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania., Munuo L; Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania., Tiambo CK; Biosciences Eastern and Central Africa-International Livestock Research Institute (BecA-ILRI) Hub, Nairobi, Kenya., Stomeo F; Biosciences Eastern and Central Africa-International Livestock Research Institute (BecA-ILRI) Hub, Nairobi, Kenya.; The European Molecular Biology Laboratory (EMBL), Heidelberg, Germany., Gwakisa P; Sokoine University of Agriculture, Morogoro, Tanzania., Mosha F; Ministry of Health Community Development Gender Elderly and Children, Dar es Salaam, Tanzania., Hudson PJ; Applied Biological and Biosecurity Research Laboratory, Pennsylvania State University, University Park, PA, USA.; The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA.; Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania.; Department of Biology, Pennsylvania State University, University Park, PA, USA., Buza JJ; Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania., Kapur V; Applied Biological and Biosecurity Research Laboratory, Pennsylvania State University, University Park, PA, USA. vkapur@psu.edu.; The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, USA. vkapur@psu.edu.; Department of Animal Science, Pennsylvania State University, University Park, PA, USA. vkapur@psu.edu.; Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania. vkapur@psu.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2021 Jul 21; Vol. 11 (1), pp. 14876. Date of Electronic Publication: 2021 Jul 21.
DOI: 10.1038/s41598-021-94112-9
Abstrakt: Meat from wildlife species (bushmeat) represents a major source of dietary protein in low- and middle-income countries where humans and wildlife live in close proximity. Despite the occurrence of zoonotic pathogens in wildlife, their prevalence in bushmeat remains unknown. To assess the risk of exposure to major pathogens in bushmeat, a total of 3784 samples, both fresh and processed, were collected from three major regions in Tanzania during both rainy and dry seasons, and were screened by real-time PCR for the presence of DNA signatures of Bacillus anthracis (B. anthracis), Brucella spp. (Brucella) and Coxiella burnetii (Coxiella). The analysis identified DNA signatures of B. anthracis (0.48%), Brucella (0.9%), and Coxiella (0.66%) in a total of 77 samples. Highest prevalence rates of B. anthracis, Brucella, and Coxiella were observed in wildebeest (56%), dik-dik (50%), and impala (24%), respectively. Fresh samples, those collected during the rainy season, and samples from Selous or Serengeti had a greater relative risk of being positive. Microbiome characterization identified Firmicutes and Proteobacteria as the most abundant phyla. The results highlight and define potential risks of exposure to endemic wildlife diseases from bushmeat and the need for future investigations to address the public health and emerging infectious disease risks associated with bushmeat harvesting, trade, and consumption.
(© 2021. The Author(s).)
Databáze: MEDLINE
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