Chrysin alleviates alteration of bone-remodeling markers in ovariectomized rats and exhibits estrogen-like activity in silico .

Autor:	Ibrahim SO; Department of Biochemistry, 58989Ahmadu Bello University, Zaria, Nigeria., Mada SB; Department of Biochemistry, 58989Ahmadu Bello University, Zaria, Nigeria., Abarshi MM; Department of Biochemistry, 58989Ahmadu Bello University, Zaria, Nigeria., Tanko MS; Department of Veterinary Surgery, 58989Ahmadu Bello University, Zaria, Nigeria., Babangida S; Department of Biochemistry, 58989Ahmadu Bello University, Zaria, Nigeria.
Jazyk:	angličtina
Zdroj:	Human & experimental toxicology [Hum Exp Toxicol] 2021 Dec; Vol. 40 (12_suppl), pp. S125-S136. Date of Electronic Publication: 2021 Jul 21.
DOI:	10.1177/09603271211033777
Abstrakt:	Background: Evidences are beginning to accrue that flavonoids, particularly phytoestrogens, could have beneficial effects against several age-related diseases linked to estrogen deficiency including postmenopausal osteoporosis. Methods: In this study, the effect of chrysin on selected bone-remodeling markers in ovariectomized rats and its estrogen-like activity in silico were investigated. Results: The data indicated that administration of chrysin at 50 mg/kg and 100 mg/kg for 6 weeks to OVX rats significantly ( p < 0.05) prevented body weight gain and partially reverse uterine weight loss. In addition, treatment of OVX rats significantly ( p < 0.01) increased femur dry weight, femur ash weight, bone ash calcium, and phosphorous levels in a dose-dependent manner. However, there was significant ( p < 0.001) decline in serum estradiol level in all OVX rats compared to the sham-operated group. Interestingly, administration of chrysin significantly ( p < 0.05) reversed the reduction of estradiol induced by ovariectomy compared to untreated OVX rats. Moreover, administration of chrysin to OVX rats significantly ( p < 0.05) suppressed excessive elevation of bone-remodeling markers expression compared to untreated OVX rats. Similarly, molecular docking analysis revealed that chrysin interacts with both α and β estrogen receptors with exothermic binding energies of -229.83 kcal/Mol and -252.72 kcal/Mol, respectively, and also fits perfectly into the active site of both α and β estrogen receptors. Conclusion: This study demonstrated that chrysin exhibits potential antiosteoporotic effects against bone loss in OVX rats through enhanced bone mineral contents and preventing excessive elevation of bone-remodeling markers and bone-resorbing cytokine.
Databáze:	MEDLINE
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