Intragenic heterochromatin-mediated alternative polyadenylation modulates miRNA and pollen development in rice.

Autor: You LY; Shanghai Center for Plant Stress Biology and Center of Excellence in Molecular Plant Sciences, Chinese Academy of Science, Shanghai, 201602, China.; University of Chinese Academy of Sciences, Beijing, 100049, China., Lin J; Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, 361102, China., Xu HW; Shanghai Center for Plant Stress Biology and Center of Excellence in Molecular Plant Sciences, Chinese Academy of Science, Shanghai, 201602, China.; College of Agriculture, Henan University of Science and Technology, Luoyang, 471023, China., Chen CX; Shanghai Center for Plant Stress Biology and Center of Excellence in Molecular Plant Sciences, Chinese Academy of Science, Shanghai, 201602, China.; University of Chinese Academy of Sciences, Beijing, 100049, China., Chen JY; Shanghai Center for Plant Stress Biology and Center of Excellence in Molecular Plant Sciences, Chinese Academy of Science, Shanghai, 201602, China.; University of Chinese Academy of Sciences, Beijing, 100049, China., Zhang J; Shanghai Center for Plant Stress Biology and Center of Excellence in Molecular Plant Sciences, Chinese Academy of Science, Shanghai, 201602, China., Zhang J; Shanghai Center for Plant Stress Biology and Center of Excellence in Molecular Plant Sciences, Chinese Academy of Science, Shanghai, 201602, China.; University of Chinese Academy of Sciences, Beijing, 100049, China., Li YX; Shanghai Center for Plant Stress Biology and Center of Excellence in Molecular Plant Sciences, Chinese Academy of Science, Shanghai, 201602, China.; University of Chinese Academy of Sciences, Beijing, 100049, China., Ye C; Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, 361102, China., Zhang H; College of Life Science, Shanghai Normal University, Shanghai, 200234, China., Jiang J; State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, 475004, China., Zhu JK; Shanghai Center for Plant Stress Biology and Center of Excellence in Molecular Plant Sciences, Chinese Academy of Science, Shanghai, 201602, China., Li QQ; Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, 361102, China.; Graduate College of Biomedical Sciences, Western University of Health Sciences, Pomona, CA, 91766, USA., Duan CG; Shanghai Center for Plant Stress Biology and Center of Excellence in Molecular Plant Sciences, Chinese Academy of Science, Shanghai, 201602, China.; University of Chinese Academy of Sciences, Beijing, 100049, China.; State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, 475004, China.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2021 Oct; Vol. 232 (2), pp. 835-852. Date of Electronic Publication: 2021 Aug 08.
DOI: 10.1111/nph.17635
Abstrakt: Despite a much higher proportion of intragenic heterochromatin-containing genes in crop genomes, the importance of intragenic heterochromatin in crop development remains unclear. Intragenic heterochromatin can be recognised by a protein complex, ASI1-AIPP1-EDM2 (AAE) complex, to regulate alternative polyadenylation. Here, we investigated the impact of rice ASI1 on global poly(A) site usage through poly(A) sequencing and ASI1-dependent regulation on rice development. We found that OsASI1 is essential for rice pollen development and flowering. OsASI1 dysfunction has an important impact on global poly(A) site usage, which is closely related to heterochromatin marks. Intriguingly, OsASI1 interacts with the intronic heterochromatin of OsXRNL, a nuclear XRN family exonuclease gene involved in the processing of an miRNA precursor, to promote the processing of full-length OsXRNL and regulate miRNA abundance. We found that OsASI1-mediated regulation of pollen development partially depends on OsXRNL. Finally, we characterised the rice AAE complex and its involvement in alternative polyadenylation and pollen development. Our findings help to elucidate an epigenetic mechanism governing miRNA abundance and rice development, and provide a valuable resource for studying the epigenetic mechanisms of many important processes in crops.
(© 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.)
Databáze: MEDLINE
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