| Autor: |
Zyrina NV; Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia., Antipova VN; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. valery_a@rambler.ru. |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2021 Jul; Vol. 86 (7), pp. 887-897. |
| DOI: |
10.1134/S0006297921070099 |
| Abstrakt: |
The review focuses on the main factors involved in the formation of nonspecific products in isothermal nucleic acid amplification, such as mispriming, ab initio DNA synthesis, and additional activities of DNA polymerases, and discusses approaches to prevent formation of such nonspecific products in LAMP, RPA, NASBA, RCA, SDA, LSDA, NDA, and EXPAR. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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