| Autor: |
Lenzi RM; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil., Campestrini LH; Edible Mushroom Cultivation Laboratory, National Institute for Amazonian Research (INPA), Manaus, AM, Brazil., Semprebon SC; Department of General Biology, State University of Londrina, Londrina, PR, Brazil., Paschoal JAR; Department of Physics and Chemistry, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil., Silva MAG; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil., Zawadzki-Baggio SF; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil., Mantovani MS; Department of General Biology, State University of Londrina, Londrina, PR, Brazil., Petkowicz CLO; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil., Maurer JBB; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, PR, Brazil. |
| Abstrakt: |
The consumption of glucosinolate (GL)-rich foods, including Brassica vegetables, such as mustard, broccoli, and maca, is associated with decreased risk of developing cancer. The GL content in maca, which is recognized as a "superfood", is approximately 100-times higher than that in other brassicas. Although maca is a potential dietary source of GLs, limited studies have examined the bioactivity of maca GLs using the combination of chemical characterization and bioassays. In this study, the fractions (Lm-II and Lm-III) rich in intact GLs (glucotropaeolin and glucolimnanthin) were isolated and characterized from maca ethanolic extracts using chromatography and mass spectrometry. Additionally, the growth-inhibitory effects of Lm-II and Lm-II fractions against hepatocellular carcinoma (HepG2/C3A) and colon adenocarcinoma (HT29) cell lines were examined in the absence or presence of myrosinase (MYR). Fractions lacking low molecular weight sugars dose-dependently exerted cytotoxic effects in the presence of MYR. The half-maximal inhibitory concentration values of Lm-II and Lm-III against HepG2/C3A were 118.8 and 69.9 µg/mL, respectively, while those against HT29 were 102.6 and 71.5 µg/mL, respectively. These results suggest that the anticancer properties of maca can be attributed to GLs and corroborate the categorization of maca as a "superfood."Supplemental data for this article is available online at https://doi.org/10.1080/01635581.2021.1952444. |
