Highly versatile antibody binding assay for the detection of SARS-CoV-2 infection.

Autor: Datta P; Public Health Research Institute, Rutgers New Jersey Medical School, Newark, NJ 07103., Ukey R; Public Health Research Institute, Rutgers New Jersey Medical School, Newark, NJ 07103., Bruiners N; Public Health Research Institute, Rutgers New Jersey Medical School, Newark, NJ 07103.; Department of Medicine, Rutgers New Jersey Medical School, Newark, NJ 07103., Honnen W; Public Health Research Institute, Rutgers New Jersey Medical School, Newark, NJ 07103., Carayannopoulos MO; Department of Pathology, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ 08901., Reichman C; Public Health Research Institute, Rutgers New Jersey Medical School, Newark, NJ 07103., Choudhary A; Public Health Research Institute, Rutgers New Jersey Medical School, Newark, NJ 07103., Onyuka A; Global Tuberculosis Institute, Rutgers New Jersey Medical School, Newark, NJ 07103., Handler D; Global Tuberculosis Institute, Rutgers New Jersey Medical School, Newark, NJ 07103., Guerrini V; Public Health Research Institute, Rutgers New Jersey Medical School, Newark, NJ 07103.; Department of Medicine, Rutgers New Jersey Medical School, Newark, NJ 07103., Mishra PK; Public Health Research Institute, Rutgers New Jersey Medical School, Newark, NJ 07103., Dewald HK; Department of Medicine, Rutgers New Jersey Medical School, Newark, NJ 07103., Lardizabal A; Global Tuberculosis Institute, Rutgers New Jersey Medical School, Newark, NJ 07103., Lederer L; Bikur Cholim of Lakewood, Lakewood, NJ 08701., Leiser AL; Cancer Institute of New Jersey, Rutgers University, New Brunswick, NJ 08903., Hussain S; Department of Medicine, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ 08901., Jagpal SK; Department of Medicine, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ 08901., Radbel J; Department of Medicine, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ 08901., Bhowmick T; Department of Medicine, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ 08901., Horton DB; Department of Pediatrics, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ 08901.; Department of Biostatistics and Epidemiology, School of Public Health, Rutgers University, Piscataway, NJ 08854., Barrett ES; Environmental and Occupational Health Sciences Institute, Rutgers University, Piscataway, NJ 08854.; Department of Biostatistics and Epidemiology, School of Public Health, Rutgers University, Piscataway, NJ 08854., Xie YL; Department of Medicine, Rutgers New Jersey Medical School, Newark, NJ 07103.; Division of Infectious Diseases, Rutgers New Jersey Medical School, Newark, NJ 07103., Fitzgerald-Bocarsly P; Department of Medicine, Rutgers New Jersey Medical School, Newark, NJ 07103., Weiss SH; Department of Medicine, Rutgers New Jersey Medical School, Newark, NJ 07103.; Cancer Institute of New Jersey, Rutgers University, New Brunswick, NJ 08903.; Department of Biostatistics and Epidemiology, School of Public Health, Rutgers University, Piscataway, NJ 08854., Woortman M; Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, NJ 08901., Parmar H; Division of Infectious Diseases, Rutgers New Jersey Medical School, Newark, NJ 07103., Roy J; Department of Biostatistics and Epidemiology, School of Public Health, Rutgers University, Piscataway, NJ 08854., Dominguez-Bello MG; Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, NJ 08901., Blaser MJ; Center for Advanced Biotechnology and Medicine, Rutgers University, Piscataway, NJ 08854., Carson JL; Department of Medicine, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ 08901., Panettieri RA Jr; Rutgers Institute for Translational Medicine & Science, New Brunswick, NJ 08901., Libutti SK; Cancer Institute of New Jersey, Rutgers University, New Brunswick, NJ 08903., Raymond HF; Department of Biostatistics and Epidemiology, School of Public Health, Rutgers University, Piscataway, NJ 08854., Pinter A; Public Health Research Institute, Rutgers New Jersey Medical School, Newark, NJ 07103.; Department of Medicine, Rutgers New Jersey Medical School, Newark, NJ 07103., Gennaro ML; Public Health Research Institute, Rutgers New Jersey Medical School, Newark, NJ 07103.; Department of Medicine, Rutgers New Jersey Medical School, Newark, NJ 07103.; Department of Biostatistics and Epidemiology, School of Public Health, Rutgers University, Piscataway, NJ 08854.
Jazyk: angličtina
Zdroj: MedRxiv : the preprint server for health sciences [medRxiv] 2021 Jul 14. Date of Electronic Publication: 2021 Jul 14.
DOI: 10.1101/2021.07.09.21260266
Abstrakt: Monitoring the burden and spread of infection with the new coronavirus SARS-CoV-2, whether within small communities or in large geographical settings, is of paramount importance for public health purposes. Serology, which detects the host antibody response to the infection, is the most appropriate tool for this task, since virus-derived markers are most reliably detected during the acute phase of infection. Here we show that our ELISA protocol, which is based on antibody binding to the Receptor Binding Domain (RBD) of the S1 subunit of the viral Spike protein expressed as a novel fusion protein, detects antibody responses to SARS-CoV-2 infection and COVID-19 vaccination. We also show that our ELISA is accurate and versatile. It compares favorably with commercial assays widely used in clinical practice to determine exposure to SARS-CoV-2. Moreover, our protocol accommodates use of various blood- and non-blood-derived biospecimens, such as breast milk, as well as dried blood obtained with microsampling cartridges that are appropriate for remote collection. As a result, our RBD-based ELISA protocols are well suited for seroepidemiology and other large-scale studies requiring parsimonious sample collection outside of healthcare settings.
Databáze: MEDLINE
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