18 F-ASEM Imaging for Evaluating Atherosclerotic Plaques Linked to α7-Nicotinic Acetylcholine Receptor.

Autor:	Yang T; Department of Cardiovascular Surgery, Fu Wai Hospital, Cardiovascular Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China., Wang D; Department of Nuclear Medicine, The Sixth Medical Center of PLA General Hospital, Beijing, China., Chen X; Department of Nuclear Medicine, The First College of Clinical Medical Sciences, China Three Gorges University, Yichang, China.; Yichang Central People's Hospital, Yichang, China., Liang Y; Department of Nuclear Medicine, The Sixth Medical Center of PLA General Hospital, Beijing, China., Guo F; Department of Nuclear Medicine, The Sixth Medical Center of PLA General Hospital, Beijing, China., Wu C; Department of Cardiovascular Surgery, Fu Wai Hospital, Cardiovascular Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China., Jia L; Department of Cardiovascular Surgery, Fu Wai Hospital, Cardiovascular Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China., Hou Z; Department of Cardiovascular Surgery, Fu Wai Hospital, Cardiovascular Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China., Li W; School of Pharmacy, Jilin Medical University, Jilin City, China., He Z; Department of Nuclear Medicine, Beijing Tsinghua Changgung Hospital, Tsinghua University, Beijing, China., Wang X; Department of Cardiovascular Surgery, Fu Wai Hospital, Cardiovascular Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Jazyk:	angličtina
Zdroj:	Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2021 Jul 01; Vol. 9, pp. 684221. Date of Electronic Publication: 2021 Jul 01 (Print Publication: 2021).
DOI:	10.3389/fbioe.2021.684221
Abstrakt:	Background: Atherosclerosis is a chronic vascular inflammatory procedure alongside with lipid efflux disorder and foam cell formation. α7-Nicotinic acetylcholine receptor (α7nAChR) is a gated-calcium transmembrane channel widely expressed in neuron and non-neuron cells, such as monocytes and macrophages, activated T cells, dendritic cells, and mast cells. 18 F-ASEM is an inhibitor targeted to α7nAChR that had been successfully applied in nervous system diseases. Previous studies had highlighted that α7nAChR was related to the emergency of vulnerable atherosclerotic plaques with excess inflammation cells. Thus, 18 F-ASEM could be a complementary diagnostic approach to atherosclerotic plaques. Materials and Methods: The synthesis of ASEM precursor and 18 F-labeling had been performed successfully. We had established the ApoE -/- mice atherosclerotic plaques model (fed with western diet) and New Zealand rabbits atherosclerotic models (balloon-sprained experiment and western diet). After damage of endothelial cells and primary plaque formation, 18 F-ASEM imaging of atherosclerotic plaques linked to α7nAChR had been conducted. In vivo micro-PET/CT imaging of ApoE -/- mice and the control group was performed 1 h after injection of 18 F-ASEM (100-150 μCi); PET/CT imaging for rabbits with atherosclerotic plaques and control ones was also performed. Meanwhile, we also conducted CT scan on the abdominal aorta of these rabbits. After that, the animals were sacrificed, and the carotid and abdominal aorta were separately taken out for circular sections. The paraffin-embedded specimens were sectioned with 5 μm thickness and stained with hematoxylin-eosin (H&E) and oil red. Results: In vivo vessel binding of 18 F-ASEM and α7nAChR expression in the model group with atherosclerosis plaques was significantly higher than that in the control group. PET/CT imaging successfully identified the atherosclerotic plaques in ApoE -/- mice and model rabbits, whereas no obvious signals were detected in normal mice or rabbits. Compared with 18 F-FDG, 18 F-ASEM had more significant effect on the early monitoring of inflammation in carotid atherosclerotic plaques of ApoE -/- mice and model rabbits. 18 F-ASEM had relatively more palpable effect on the imaging of abdominal aorta with atherosclerosis in rabbits. H&E and oil red staining identified the formation of atherosclerotic plaques in model animals, which provided pathological basis for the evaluation of imaging effects. Conclusion: We first confirmed 18 F-ASEM as radiotracer with good imaging properties for precise identification of atherosclerotic diseases. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be constructed as a potential conflict of interest. (Copyright © 2021 Yang, Wang, Chen, Liang, Guo, Wu, Jia, Hou, Li, He and Wang.)
Databáze:	MEDLINE
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