Rapid SARS-CoV-2 variant monitoring using PCR confirmed by whole genome sequencing in a high-volume diagnostic laboratory.
Autor: | Lind A; Department of Microbiology, Oslo University Hospital, Oslo, Norway., Barlinn R; Department of Microbiology, Oslo University Hospital, Oslo, Norway., Landaas ET; Department of Microbiology, Oslo University Hospital, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway., Andresen LL; Department of Microbiology, Oslo University Hospital, Oslo, Norway., Jakobsen K; Department of Microbiology, Oslo University Hospital, Oslo, Norway., Fladeby C; Department of Microbiology, Oslo University Hospital, Oslo, Norway., Nilsen M; Department of Microbiology, Oslo University Hospital, Oslo, Norway., Bjørnstad PM; Department of Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway., Sundaram AYM; Department of Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway., Ribarska T; Department of Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway., Müller F; Department of Microbiology, Oslo University Hospital, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway., Gilfillan GD; Department of Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway., Holberg-Petersen M; Department of Microbiology, Oslo University Hospital, Oslo, Norway. |
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Jazyk: | angličtina |
Zdroj: | Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [J Clin Virol] 2021 Aug; Vol. 141, pp. 104906. Date of Electronic Publication: 2021 Jul 07. |
DOI: | 10.1016/j.jcv.2021.104906 |
Abstrakt: | Objectives: The emerging SARS-CoV-2 variants of concern (VoC), B.1.1.7, B.1.351 and P.1, with increased transmission and/or immune evasion, emphasise the need for broad and rapid variant monitoring. Our high-volume laboratory introduced a PCR variant assay (Variant PCR) in January 2021 based on the protocol by Vogels et al. STUDY DESIGN: To assess whether Variant PCR could be used for rapid B.1.1.7, B.1.351 and P.1 screening, all positive SARS-CoV-2 airway samples were prospectively tested in parallel using both the Variant PCR and whole genome sequencing (WGS). Results: In total 1,642 SARS-CoV-2 positive samples from individual patients were tested within a time span of 4 weeks. For all samples with valid results from both Variant PCR and WGS, no VoC was missed by Variant PCR (totalling 399 VoC detected). Conversely, all of the samples identified as "other lineages" (i.e., "non-VoC lineages") by the Variant PCR, were confirmed by WGS. Conclusions: The Variant PCR based on the protocol by Vogels et al., is an effective method for rapid screening for VoC, applicable for most diagnostic laboratories within a pandemic setting. WGS is still required to confirm the identity of certain variants and for continuous surveillance of emerging VoC. (Copyright © 2021. Published by Elsevier B.V.) |
Databáze: | MEDLINE |
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