Bread Wheat Doubled Haploid Production by Anther Culture.

Autor:	Castillo AM; Department of Genetics and Plant Production. Aula Dei Experimental Station, Spanish National Research Council (EEAD-CSIC), Avda. Montañana 1005, Zaragoza, Spain. amcast@eead.csic.es., Valero-Rubira I; Department of Genetics and Plant Production. Aula Dei Experimental Station, Spanish National Research Council (EEAD-CSIC), Avda. Montañana 1005, Zaragoza, Spain., Allué S; Department of Genetics and Plant Production. Aula Dei Experimental Station, Spanish National Research Council (EEAD-CSIC), Avda. Montañana 1005, Zaragoza, Spain., Costar MA; Department of Genetics and Plant Production. Aula Dei Experimental Station, Spanish National Research Council (EEAD-CSIC), Avda. Montañana 1005, Zaragoza, Spain., Vallés MP; Department of Genetics and Plant Production. Aula Dei Experimental Station, Spanish National Research Council (EEAD-CSIC), Avda. Montañana 1005, Zaragoza, Spain.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2287, pp. 227-244.
DOI:	10.1007/978-1-0716-1315-3_11
Abstrakt:	The use of doubled haploid (DH) plants in plant breeding programmes is the fastest route to release new varieties (4-6 years), allowing for a rapid response to end-user needs. Microspore embryogenesis is one of the most efficient methods for DH plant production in bread wheat. In this process, microspores triggered by a stress treatment or by application of bioactive compounds are reprogrammed to follow an embryogenic pathway that leads to the production of haploid or DH plants. In this chapter, we describe a protocol for anther culture of bread wheat. This protocol is based on an osmotic and starvation treatment of the anthers followed by the application of a microtubule disrupting agent. Anthers are cultured in an ovary pre-conditioned medium with mature ovaries from cv. Caramba. This protocol has been applied to a wide range of genotypes and F1s from bread and spelt wheat. (© 2021. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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